The knocked-in CAG repeat expansion in Q140 mice and the consistent expression of the mutantHttgene and protein in the brain were important reasons to initially test the therapeutic potential of G58TF EVs in these mice. However, slow progression of disease symptoms (>12 months) in Q140 mice...
Mice lacking the GAPDH redox switch exhibit altered fatty acid metabolism in kidney and heart, apparently in compensation for the lack of the redox switch. Together, our findings demonstrate the physiological and pathophysiological relevance of oxidative GAPDH inactivation in mammals....
on an abi prism 7300 sequence detection system. the data were analyzed by the delta-delta ct method. the expression level of each gene was normalized with actin rna. primers are listed in additional file 2 : table s7. protein extraction for mass spectrometry analysis protein extraction was ...
In vivo, phenformin treatment increases the survival of Eμ-Myc-GAPDHlow lymphoma-bearing mice, but not that of Eμ-Myc-GAPDHhigh lymphoma-bearing mice (Figures 2F and 2G), which demonstrates the causal correlation between the low expression of GAPDH and phenformin sensitivity. We confirmed ...
An anti-GAPDH antibody can induce high level of bactericidal capability; a gapdh gene has a transcription level in SEZ infected mice much higher than that of culture in vitro; and the rGAPDH can significantly weaken cell adhesion ability of the SEZ, and greatly reduce cell infection ability of...
Its potential as a contraceptive target was supported by data from mice where GAPDS−/− males (23) were infertile because of defects in sperm motility. Glyceraldehyde-3-phosphate dehydrogenases are tetrameric enzymes that catalyze the oxidative phosphorylation of d-glyceraldehyde 3-phosphate (Glc...
Primers are listed in Addi- tional file 2: Table S7. Protein extraction for mass spectrometry analysis Protein extraction was done essentially as described pre- viously. Cells were resuspended in 400 μl of lysis buffer (8 M urea and cocktail protease inhibitors (Sigma), 20 ...
Over-activation of AMPARs (α−amino-3-hydroxy-5-methylisoxazole-4-propionic acid subtype glutamate receptors) is implicated in excitotoxic neuronal death associated with acute brain insults, such as ischemic stroke. However, the specific molecular mec
The invention relates to a fluorescence quantitative polymerase chain reaction kit which is simple and fast to operate and can realize effective quantitative amplification detection of GAPDH genes of human beings, rats and mice. The kit comprises a PCR amplification buffering reaction system composed of...
mice with lethal endotoxaemia. IL-1β is the principal pro-inflammatory cytokine produced in response to infectious insults, and IL-6 is one of the main indicators of patients with sepsis48,49. 4-OI conferred significant protection against lethal endotoxaemia in mice, partly by its significant ...