上海玉博生物科技有限公司 主要致力于“[pSer139]Histone gamma-H2AX (human), pAb ALX-210-390-R050”的生产销售。多年的“[pSer139]Histone gamma-H2AX (human), pAb ALX-210-390-R050”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深
The permeabilization of the antibody in the cell was used with 1 mL of pre-chilled ethanol 70% (−20 °C) for 20 min. The washing stage was done again. Without any agitation, the cells were incubated with 10 μL of anti-gH2AX antibody (Cell Signalling Tech., USA) at 400 × ...
Primary antibody incubations with anti- gH2AX or with anti-CK2a' were performed for 40 min at 37°C at 1:500 dilution (2 μg/ml)) in PBS supplemen- ted with 2% bovine serum fraction V albumin (Sigma- Aldrich) and followed by washing 4 times in PBS. Incu- bations with fluoro...
Primary antibodies against Wip1 (CST, United States), H2AX (CST), γ-H2AX (CST) and β-actin (Sigma, United States) were used. Super Signal West Dura Extended Duration Substrate (Pierce, German) was used to detect antibody-antigen complexes. Drug administration To intervene the progression ...
Histone H2A.x 组蛋白H2AX抗体规格: 0.2ml presenilin 1/F42 Mdivi 1 (50 mg)Mitochondrial Division Inhibitor 1; 3-(2,4-dichloro-5-methoxyphenyl)-2,3-dihydro-2-thioxo-4(1H)-quinazolinone Hoechst 33342 (hydrochloride) (500 mg)Bisbenzimide|HOE 33342|NSC 334072; 2’- Calpeptin (25 mg)...
Exhibitor Profile - Sigma Gamma Epsilon
The irradiated cells were fixed, permeabilized, and stained with an anti-γ-H2AX antibody and DAPI. γ-H2AX was visualized as green, and DAPI was visualized as blue. The images of stained cells were acquired and analyzed. (e) Effects of PGE2 treatment after irradiation on DNA DSBs resulting...
To support the results of the neutral comet assay, the γ-H2AX assay was also performed on PSHE pretreated splenocytes (50 µg/mL) after 15 min of 4 Gy gamma irradiation, as described earlier [39]. After immunostaining, the fluorescence intensity was monitored using a high throughput ...
Quantitative detection of 125IdU-induced DNA double-strand breaks with γ-H2AX antibody. Radiat. Res. 2002, 158, 486–492. [Google Scholar] [CrossRef] Leatherbarrow, E.L.; Harper, J.V.; Cucinotta, F.A.; O’Neill, P. Induction and quantification of γ-H2AX foci following low and high...
(r2> 0.99). However, the single or integrated dose–response curves of these genes were distinctive from the conventional models obtained by the gamma-H2AX or comet assays. In comparison, rice displayed a higher dose-dependency in the comet signal andOsRAD51transcription, while the gamma-H2AX ...