With the help of a Drosophila geneticist, a series of crosses can be done to combine the GAL4 line, the mutant allele, and the UAS-cDNA into a single fly. Just as in the Trojan-GAL4 strategy, if the wild-type UAS-cDNA is able to rescue the mutant phenotype but the disease-...
The red flour beetle Tribolium castaneum has developed into an insect model system second only to Drosophila. Moreover, as a coleopteran it represents the most species-rich metazoan taxon which also includes many pest species. The genetic toolbox for Tribolium research has expanded in the past yea...
geneshavebeenestablished.Whenexpressedindividuallybycrossingtoanarrayof6differentGAL4driverlines,oneofthesegenes,thetranslationelongationfactor1α1(EF1α21),resultedinabnormalnotumandrougheyephenotypes.ThisstudyimpliesthefeasibilityofsystematicallyscreeninghumangenefunctionsbyoverexpressioninDrosophila.GAL42UASphenotype;...
However, in the presence of protein interactions, the ADE2 marker complements in cis the AH109 ade2-101 phenotype. b. When transformed with pAS2-1, or any pAS2-derived plasmid, CG-1945 grows more slowly and forms noticeably smaller colonies than untransformed CG-1945. 3. Antibiotic ...
clonetech公司 Gal4系统酵母双杂交与筛库.docx,Matchmaker?GAL4Two-HybridSystem3LibrariesUserManualUnitedStates/Canada800.662.2566AsiaPaci?c+1.650.919.7300Europe+33.(0)1.3904.6880Japan+81.(0)77.543.6116PT3247-1(PR742219)Published3April2007ClontechLaboratorie