Beyond the knowledge pertaining to the binding of Gal4 on the promoter to initiate gene expression, limited information exists regarding the structure of the GAL1/GAL10 promoter sequence and how that structure mechanistically leads to the initiation of gene expression. The promoter contains 688 base...
基因 种属 Human NCBI 参考序列号 NM_198321.3 参考序列ORF长度 1812 bp 描述 Full length Clone DNA of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10). 质粒 启动子 Enhanced CMV promoter
The single intrinsic DNA bend on the GAL80 promoter lies in the non-nucleosomal region, between the UASGAL80 and the more gene-proximal promoter elements (Fig. 6). The GAL80-specific UASGAL80 activation sequence is the most upstream element on GAL80 and is required for both basal and indu...
All of the combinations showed no apparent coordinated promoter function under our experimental conditions; 3) Sequences between -89bp and -41bp of SUC 2 upstream region are responsible for constitutive expression of nonglycosylated invertase. The two tracts of poly (dA-dT) of this region may ...
The upstream of GALNT10 gene was then cloned into a promoter-free luciferase reporter construct. Luciferase assays demonstrated that BAG3 knockdown significantly decreased the luciferase activity of construct containing GALNT10 promoter (Fig. 2N). These data indicated that BAG3 regulated GALNT10 ...
galP1 promoterTranscriptionMost Escherichia coli promoters are consists of two 6-bp sequence elements which are centered at ~10 and 35 nucleotides upstream of the transcription initiation point. The conserved region 4.2 and 2.4 of the RNA polymerase σ~(70) subunit recognize -10 and -35 promoter...
Sequences that regulate the divergent UGAL1u-UGAL10u promoter in USaccharomycesu UcerevisiaeuJohnston, MDavis, R W
On some promoters, the presence of RNAP α-subunit C-terminal domains (αCTDs) greatly increases transcription initiation beyond the basal level achieved through σ70-promoter element interactions (4). On these promoters, αCTDs make sequence-specific interactions with an A-rich promoter element ...
2. The hybrid yeast promoter according to claim 1, wherein the upstream activation sequence of the Saccharomyces cerevisiae PGK promoter sequence has, at the same location, been substituted with the upstream activation sequence of the Saccharomyces cerevisiae GAL10 gene. ...
promotergenomic structurehousekeeping geneGC-rich regionSwiss 3T3Nucleosome structure and repair of N-methylpurines were analyzed at nucleotide resolution in the divergent GAL1-10 genes of intact yeast cells, encompassing their common upstream-activating sequence. In glucose cultures where genes are ...