Because transcription from galactose-controlled promoters is absolutely dependent upon GAL4 protein, the maximal level of expression from a galactose-controlled promoter is limited by level of the GAL4 protein in the cell. For instance, Klar and Halvorson (1976) have shown that the level of synt...
Expression of your gene of interest from the inducible expression vector is controlled by the strong CMV promoter (Andersson et al., 1989; Boshart et al., 1985; Nelson et al., 1987) into which 2 copies of the tet operator...
An inducible expression plasmid, pGene/V5-His, for expression of your gene of interest under the control of a hybrid promoter containing GAL4 upstream activating sequences (UAS) and the adenovirus E1b TATA bo...
3A, we also employed the layered design to reprogram the allantoin-inducible system and created an allantoin–GAL switch. Firstly, we deleted the Gal80 gene in BY4741 strain, which acts as a negative regulator of the Gal4 activator. Subsequently, we substituted the promoter of the Gal4 gene...
Traditionally, regulated gene expression in Drosophila cultured cell lines has been achieved by placing coding sequences of interest under direct control of a constitutive promoter, such as pAct5C from the actin5C gene (e.g., Krasnow et al., 1989), or an inducible promoter, such as pMT ...
30 The Gal4 transcriptional activator regulates transgene expression under upstream activating sequence (UAS) promoter, which is a widely used expression system in Drosophila.31., 32., 33. Despite much progress with the existing Gal4-UAS system, there is still a need to improve its functionality...
For this analysis, we used the mammalian two-hybrid system, which contains Gal4 promoter-luciferase, Gal4-CAD, and VP16-CH1 plasmids. Consequently, the CAD–CH1 interaction was markedly enhanced by NDRG3 knockdown but almost completely inhibited by NDRG3 overexpression (Fig. 5c), strongly ...
To this end, we intercrossed the MV-Dronc-V5-VC founder strain with flies expressing FlpWT under the regulation of a βTubulin 85D-promoter (βTub85D-FlpWT, hereafter; this fly strain was kindly provided by Professor Konrad Basler) (Fig. 3a). The activity of βTubulin 85D-...
These results indicate that the DAO1-in1 promoter is a useful tool for inducible expression of genes under lipid production conditions, particularly when a DAO1 null mutant is used. The strength of gene expression could be tuned by adjusting the levels of inducer, carbon source and nitrogen ...
Mutations in the AXR3 gene of Arabidopsis result in altered auxin response including ectopic expression from the SAUR-AC1 promoter. Plant J 1996; 10:403-413. 44 Tian Q, Reed JW. Control of auxin-regulated root development by the Arabidopsis thaliana SHY2/IAA3 gene. Development 1999; 126:...