(2013). Gain-of-function assays in the axolotl (Ambystoma mexicanum) to identify signaling pathways that induce and regulate limb regeneration. Meth Mol Biol, 1037, 401-17.Lee J, Aguilar C, Gardiner D (2013) Gain-of- function assays in the axolotl (Ambystoma mexicanum) to identify signaling...
8a). Colony-formation assays also revealed no significant differences for MCF10A and CAL-51 cells by TP53 genotype or with p53 knockdown; however, cell lines with the most colonies formed were among those with the highest AS (M1-33, C2-56, Fig. 4c, d), indicating that changes in cell ...
(g) Representative histograms of CFSE dilution assays of anti-CD3/anti-CD28 activated CD4+ and CD8+ T cells from a HC and a PPBL patient. Representative of 3 independent experiments. (h) T cell reactivity against CMV peptides (pp65 and IE-1) between HCs (n=2) and PPBL patient T ...
Densitometry analysis results for the phosphorylation and de-phosphorylation assays above were graphed and are presented in panels c and d, respectively. abnormal pattern of ISG expression even in "naive" cells prior to cytokine stimulation. Taken together, our current findings support the notion that...
Most known pathogenic mutations occur in protein-coding regions of DNA and change the way proteins are made. Taking protein structure into account has therefore provided great insight into the molecular mechanisms underlying human genetic disease. While
(C-H) The data obtained to use cell counting kit-8 assays revealed that p53R273H (F, H) and shp53 (E, G) had a significant effect on the proliferation of wild-type p53 cells (HCT116) under normal condition.To explore the higher oncogenic potential caused by mutant p53R273H in ...
(A) Schematic depiction of the experimental schemes for the Förster resonance energy transfer (FRET)-based assays. Binding of RPA:RPA1WT (RPAWT), RPA:RPA1V227A (RPAV227A), RPA:RPA1V270A (RPAV270A), and RPA:RPA1E240K (RPAE240K) proteins was monitored by using 1 nM dT30 ssDNA...
Mutation-specific dPCR assays demonstrated decreased frequencies of the SAMD9L mutations in DNA from the myelodysplastic bone marrow of F1:I-3 and F2:II-4, indicating that the loss of chromosome 7 material eliminated the SAMD9L mutations (Figure 3C-D). Seemingly, the SAMD9L mutations were not...
In vitro histone acetylation assays have revealed that together, the two point mutations cause a sevenfold increase in the histone acetyltransferase (HAT) activity of recombinant CBP-1. To our knowledge, this is the only such HAT activity mutation isolated in a CBP/p300 family protein, and this...
Assays were performed at 251C in the absence of allosteric effectors. Each point represents the mean±SEM (bar) of three to five experimental determinations. The slope (±SE) of each regression line is given below as the rate of change of specific activity (in mmol/min/mg) per mg ...