07 June 2016 Sulfation of the FLAG epitope is affected by co-expression of G protein-coupled receptors in a mammalian cell model Morag Rose Hunter†, Natasha Lillia Grimsey & Michelle Glass G protein-coupled receptors (GPCRs) are important therapeutic targets and therefore extensively...
G protein-coupled receptor overexpression with the baculovirus-insect cell system: a tool for structural and functional studies. Biochim. Biophys. Acta 1610, 77–89 (2003). 25. Macauley-Patrick, S., Fazenda, M. L., McNeil, B. & Harvey, L. M. Heterologous protein production using the ...
V. G protein-coupled receptor kinases: more than just kinases and not only for GPCRs. Pharmacol. Ther. 133, 40–69 (2012). 2. Qanbar, R. & Bouvier, M. Role of palmitoylation/depalmitoylation reactions in G-protein-coupled receptor function. Pharmacol. Ther. 97, 1–33 (2003). 3. ...
www.nature.com/scientificreports OPEN Thermostability of a recombinant G protein‑coupled receptor expressed at high level in mammalian cell culture Alexei Yeliseev1*, Arjen van den Berg2, Lioudmila Zoubak1, Kirk Hines1, Sam Stepnowski2, Kyle Williston2, Wanhua ...
Protein kinase A (PKA) appears to be a crucial player in these events, because activation of progesterone receptor is followed by a sudden drop in cAMP concentration (11) and likely a subsequent inactivation of protein kinase A. Indeed, microinjection of the PKA catalytic subunit prevents ...
Gertzen2, Lina Spomer3, Mykola Dimura1, Thomas O. Peulen1, Christina Wöhler3, Dieter Häussinger3, Holger Gohlke2, Verena Keitel3 & Claus A. M. Seidel1 TGR5 is the first identified bile acid-sensing G-protein coupled receptor, which has emerged as a ...
Alamandine (Ala), a ligand of Mas-related G protein-coupled receptor, member D (MrgD), alleviates angiotensin II (AngII)-induced cardiac hypertrophy. However, the specific physiological and pathological role of MrgD is not yet elucidated. Here, we found
et al., “Human herpesvirus KSHV encodes a constitutively actige G-protein-coupled receptor agonist activity of receptor ligands,” J. Biol. Chem. 1994, 269(16), 11687-11690. Barker, E.L. et al., “Constitutively active 5-hydroxytryptamine 2c receptors reveal novel inverse agonist activity...
The hydrophilic side of each G-protein coupled receptor transmembrane helix is postulated to face inward and form polar ligand binding site. TM3has been implicated in several G-protein coupled receptors as having a ligand binding site, such as the TM3 aspartate residue. TM5 serines, a TM6 ...
A human G-protein coupled receptor polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also di