doi:10.1002/0471140864.ps2914s81cell‐free expressionE. coli extractmembrane proteinGPCRdetergentlipidSegers, KennethMasure, StefanJohn Wiley & Sons, Inc.
G protein-coupled receptors signaling Synaptic transmission can be classified into two types: fast and slow synaptic transmission.23 In fast synapses, GPCRs such as glutamate and GABA (γ-aminobutyric acid) receptors generate membrane depolarizing signals in less than 1/1000 s. In slow synapses,...
G protein-coupled receptor kinases (GRKs) phosphorylate activated G protein-coupled receptors, leading to their desensitization and endocytosis. GRKs have also been implicated in phosphorylating other classes of proteins and can localize in a variety of cellular compartments, including the nucleus. Here...
G protein-independent, arrestin-dependent signaling is a paradigm that broadens the signaling scope of G protein-coupled receptors (GPCRs) beyond G proteins for numerous biological processes. However, arrestin signaling in the collective absence of functional G proteins has never been demonstrated. Here...
A nuclear magnetic resonance (NMR) study of Gαi1 indicates that GDP-bound Gα subunits dynamically exist in two conformational states: a ground state that is preferred by G protein-coupled receptors (GPCRs) and an excited state that is similar to the GTP-bound state (Goricanec et al., ...
BRET技术在G蛋白偶联受体和β- arrestins相互作用研究中的应用进展 杜辉 , 2,白波3* ,陈京1泰山医学院神经生物学研究所,泰安271000;山东农业大学生命科学院, 泰安271000; 济宁医学院神经生物学研究所,济宁272000 [摘要] G蛋白偶联受体(G protein-coupled receptor, GPCR)是一个重要的细胞膜受体超家族。β-arresti...
With the nationwide introduction of wastewater treatment the overall water quality improved significantly, but challenges remain, including diffuse pollution, historical sediment contamination and the presence of a multitude of anthropogenic chemical spe
5.19N, QH5.22E, EH5.24N, and LH5.26V), as well as by changing the N-terminus with that of Gαi. The resulted miniGs/q70iNconstruct recognizes the Gq-coupled receptors26and reserves the interaction interface of nanobody35 (Nb35). This miniGs/q70iNfusing strategy improved the protein ...
Cryoelectron microscopy G protein-coupled receptors This article is cited by Trace amine associated receptor 1: predicted effects of single nucleotide variants on structure-function in geographically diverse populations Britto Shajan Shashikanth Marri Pramod C. Nair Human Genomics (2024) Dopamine D1...
The macromolecule targets were: a B-DNA (PDB: 1BNA) and caspase 3 protein (PDB: 3KJF). These two macromolecular receptors have been chosen according to the experimental finding, which declared that the complex has the best effect on the action of DNA and caspase 3 activity (vide supra)....