FXR floxed mice (Fig.2c). To activate FXR signaling, the mice were briefly fed with CA chow before killing. Hepatic p-Y67-FXR levels were restored to the levels similar to those in the FXR-floxed mice by expression of FXR-WT, but not in mice expressing Y67F-FXR mutant (Fig.2c)....
c Clinical score of day-time and night-time fed FxrΔHep (solid line) or Fxr-floxed control female mice (dotted line) following 20 mg/kg LPS i.p. stimulation at ZT0 on day 5 of TRF. Statistical analysis using two-way ANOVA and Sidak’s multiple comparison. FxrΔHep vs. Fxrfl/...
Six-week-old male C57BL/6J mice were purchased from Jackson Laboratory or Charles River Laboratories. iScarb1−/− mice were generated by mating floxed SR-B1 (Scarb1fl/fl) mice42 with Villin-Cre mice (Jackson Laboratory, Stock#021504). The method for creating Scarb1fl/fl mice has ...