The novel transmembrane aspartic protease BACE (for Beta-site APP Cleaving Enzyme) is the beta-secretase that cleaves amyloid precursor protein to initiate beta-amyloid formation. As such, BACE is a prime therapeutic target for the treatment of Alzheimer's disease. BACE, like other aspartic ...
1B and Table 1). In the conserved replicase domains, Bat CoV CD35 shared 68.3%–91.5% aa identity in the 3C-like protease (3CLpro) region, 77.0%–93.1% in the RdRp region, and 81.0%–89.2% in the helicase (Hel) region with the corresponding sequences of Bat Hp-betaCoV Zhejiang...
proteinasecleavagesites.CellsexpressingCFRweretreatedwiththefurinproteaseinhibitordecanoyl- Arg-Val-Lys-Arg-chloromethylketone(decCMK)ortheMMP-inhibitorGM6001.Inthepresenceof furin-likePCinhibitor,secretionofCFRwasalmostcompletelyinhibited.Secretionwasnotaffectedby ...
Fresh cell pellets were washed twice in milliQ H2O before being ground in 100 μl lysis buffer (50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2.5 mM EDTA, 0.2% Triton-X, 5% glycerol, complete EDTA free protease inhibitor cocktail (Roche)). For immunoblotting of embryos,∼50 μ...
It was also demonstrated that HIV infection induces the expression of either the protease activated receptor 1 (PAR1) (Kim et al., 2015) or guanylate binding proteins 2 and 5 (GBP2,5) (Braun and Sauter, 2019) that restrict the trafficking of furin to the trans Golgi network (PAR1) ...
To investigate whether or not alternative splicing might be a mechanism by which in Drosophila melanogaster diversity is generated in endoproteases of the novel eukaryotic family of subtilisin-like proprotein processing enzymes, we determined structural and functional characteristics of the Dfur1 gene. ...
Phospholipase Protease Angiopoietin-like 3 Endothelial Lipase Furin Knockout Mice Proprotein Convertases Introduction A large number of secretory proteins are produced as precursors that are cleaved at specific sites to generate mature bioactive products. Most of these specific cleavages occur after basic...
LUEBKE , DAVID L. WILSON , IAN M. DICKERSON . (1997) Isolation of a cDNA Encoding a Kex2-Like Endoprotease with Homology to Furin from the Nematode Caenorhabditis elegans . DNA and Cell Biology 16 , 663-669 Online publication date: 1-May-1997. /...
Protease inhibitorFurinSubtilaseMechanism-based inhibitionRecent studies have demonstrated that a serpin variant, α1-antitrypsin Portland (AT-PDX), can inhibit the mammalian convertase furin. Here, we examine the mechanism by which this inhibition takes place. We find that furin, which does not ...