Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC), and dihydrofolate reductase (DHFR) in one transcript were designed to compare internal ribosome entry site (IRES) and furin-2A (F2A) for their influence on monoclonal antibody (mAb) expression level...
Jostock T, Dragic Z, Fang J, Jooss K, Wilms B, Knopf HP (2010) Combination of the 2A/furin technology with an animal component free cell line development platform process. Appl Microbiol Biotechnol 87(4):1517–1524THOMAS JOSTOCK ET AL: "Combination of the 2A/furin technology with an ...
(2011). Stable expression of native Coagulation factor VIII using the 2A self-processing sequence and furin cleavage site. Thromb. Res. 128, e148-e153. doi: 10.1016/j.thromres.2011. 07.015He B; Pan Y; Chen L;.Stable expression of native eoag- ulalion factor Ⅷ using the 2A self-...
目的观察口腔鳞癌术后组织中细胞纤毛转运蛋白(IFT)20,弗林蛋白酶(Furin)和上皮型黏附素(E-cadherin)的表达特征,分析其临床意义.方法 112例口腔鳞癌术后组织作为研究组,以60例距肿瘤边缘3 cm的正常牙龈黏膜组织作为对照组,采用免疫组化SP法检测两组中IFT20,Furin和E-cadherin的表达特征.结果两组中IFT20,Furin和E...
目的:检测胰腺导管腺癌患者癌组织中切除修复交叉互补基因(ERCC)1,Furin和10号染色体缺失 的磷酸酶及张力蛋白同源物(PTEN)的表达.方法78例胰腺导管腺癌作为观察组,以50例胰腺上皮性良性肿瘤(25例浆液性囊腺瘤和25例黏液性囊腺 瘤)作为对照组.采用免疫组织化学方法检测ERCC1,Furin和PTEN的表达及三者在不同临床病理...