Jostock T, Dragic Z, Fang J, Jooss K, Wilms B, Knopf HP (2010) Combination of the 2A/furin technology with an animal component free cell line development platform process. Appl Microbiol Biotechnol 87(4):1517–1524THOMAS JOSTOCK ET AL: "Combination of the 2A/furin technology with an ...
Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC), and dihydrofolate reductase (DHFR) in one transcript were designed to compare internal ribosome entry site (IRES) and furin-2A (F2A) for their influence on monoclonal antibody (mAb) expression level...
product in the Trk:Ch cell supernatant (Fig. 1dandSupplementary Fig. 2a) and a significant reduction in the amount of cleavage product in the NTrk:Ch cell supernatant (Fig. 1eandSupplementary Fig. 2b). These data suggest that Furins cleave Trk in S2 cells. ...
图1. Furin蛋白酶和各抗病毒蛋白的模式图 图2A. GBP2/5 抑制Furin蛋白酶切割 HIV-1 糖蛋白的机制 图3A. MARCH8 抑制 EBOV GP 的切割和糖基化成熟 图4A. -SNAP抑制 SARS-CoV-2 S 糖蛋白裂解的机制 图5A. PAR1 抑制 HIV-1 糖蛋白裂解的机制 郑春福教授,入选Elsevier的2022年全球Top2%顶尖科学家榜单,...
2A). Considering PTENα/β fragments were only detected in xenografts but not in in vitro-cultured cell lysates, we reasoned that the cleavage might specifically take place in the extracellular space. To test this, bacterially purified TrxA-S-tag-PTENα-Flag together with TrxA-S-tag-Flag as...
Band in lane 4 has a vertical, straight right edge. Figure 1D. Lower bands in lanes 3 and 5 have vertically truncated left ends. Figure 2. http://www.pnas.org/content/98/18/10326/F2.large.jpg Figure 2A. Vertical change in background between lanes 2 and 3. ...
As demonstrated in Figure 3A, lane 2 (also shown in Figure 2A), BSC-40 cells infected with vaccinia encoding proTGF-β1 produced ∼50% of processed TGF-β as seen by the relative intensity of the pro-region and proTGF-β1 bands. Co-infection of cells with 1 to 6 multiplicity of ...
NM002569-9 pCMV-Tag 2A 全长 N-Flag 哺乳细胞 2800 NM002569-10 pCMV-Tag 4A 全长 C-Flag 哺乳细胞 2800 NM002569-11 pCMV-HA 全长 N-HA 哺乳细胞 2800 NM002569-12 pEGFP-C1 全长 N-EGFP 哺乳细胞 2800 NM002569-13 pEGFP-N1 全长 C-EGFP 哺乳细胞 2800 NM002569-14 p3xFlag-CMV-10 全长 ...
In this project, the natural product libraries of Med Chem Expression and COCONUT [40], and the crystal structure of furin (PDB ID: 4Z2A) were used for virtual screening. Firstly, LeDock was employed as a rapid screening tool for the initial screening of 200,000 compounds, the compounds ...
2A) or transiently transfected mouse ANGPTL3 (Fig. 2, B–D) in either wild type (WT) primary hepatocytes, or in those lacking specifically PC5/6 (PC5/6-hKO), PACE4 (PACE4-KO), or furin (Fur-hKO). The data show that the percentage of endogenous secreted CT forms was not affected...