ELISA procedures were executed following the manufacturer’s instructions. Six independent samples per group were used. Extraction of total protein and Western blot analysis Complete A. thaliana plantlets cultured for 1, 7, and 14 days were used to prepare total proteins. Total proteins were ...
japonica was extracted according to the instructions of the DP43 kit, and cDNA was synthesized with the FastKing RT Kit, a reverse transcription kit. The cDNA template was added to the reaction system by real-time PCR using an ABI7500 instrument. The reaction system included 10 µL of ...
Western blot. Cell lysates were prepared from motile sperms using RIPA buffer according to the manu- ®facturer's instructions (Beyotime, China). Fifty-six micrograms of denatured human sperm total proteins were separated on a 10% SDS-PAGE gel with a Criterion system (Bio-Rad, USA)...
Real-time PCR was performed using an Applied Biosystems 7500 Real-time PCR System. KAPA SYBR® FAST qRT-PCR Kit was used according to the manufacturer’s (Kapa Biosystems, Cape Town, South Africa) instructions. The programme for the PCR reactions was: 50°C for 2 minutes; 95°C for 10...
Total RNA was extracted using the RNAprep Pure Plant Kit (Tiangen) according to the manufacturer’s instructions and quantified with NanoDrop spectrophotometer. RNA-seq libraries were constructed using the Illumina TruSeq Stranded mRNA Library Preparation Kit using 1–2 μg of total RNA and were the...
(KCNK3, NM_002246), TRAAK (KCNK4, AF247042), TWIK-1 (KCNK1, U33632), TASK-3 (KCNK9, AF212829), TREK-1b (KCNK2, AF004711) or TASK-4 (KCNK17, AF358910) cDNA were introduced with the QuickChange Site Directed Mutagenesis Kit (Stratagene) according to the instructions of ...
PACS1 syndrome is a neurodevelopmental disorder characterized by intellectual disability and distinct craniofacial abnormalities resulting from a de novo p.R203W variant in phosphofurin acidic cluster sorting protein 1 (PACS1). PACS1 is known to have fun
ABA: abscicic acid ACC: 1-aminocyclopropane-1-carboxylic-acid AHA: Aromatic, large Hydrophobic, Acidic context CST: complete sequence tree CTR: carboxy-terminal region ET: ethylene HALZ: homeodomain-associated leucine zipper HD-Zip: homeodomain-leucine zipper HSF: Heat Stress Transcription...
TxshmceoelRltehhcosttdeemdrewfpraoosmrtmepdelabasnyutSrsaescdukbuejsteiacnltg.e6d6a, to water stress at the end of the drought treatment by a transient-pressure method, following the manufacturer's instructions. Scientific Reports | 6:20167 | DOI: 10.1038/srep20167 12 www.nature.com/...
Complete plasmid sequences were retrieved from the PLSDB v.20210623_v290, and Mash v.2.391 sketches were generated following the instructions on the PLSDB website. Plasmid sequences were screened from our assembled contigs using Mash dist, with the Mash distance < 0.001. Plasmids were annota...