ELISA procedures were executed following the manufacturer’s instructions. Six independent samples per group were used. Extraction of total protein and Western blot analysis Complete A. thaliana plantlets cultured for 1, 7, and 14 days were used to prepare total proteins. Total proteins were ...
japonica was extracted according to the instructions of the DP43 kit, and cDNA was synthesized with the FastKing RT Kit, a reverse transcription kit. The cDNA template was added to the reaction system by real-time PCR using an ABI7500 instrument. The reaction system included 10 µL of ...
Total RNA was extracted using the RNAprep Pure Plant Kit (Tiangen) according to the manufacturer’s instructions and quantified with NanoDrop spectrophotometer. RNA-seq libraries were constructed using the Illumina TruSeq Stranded mRNA Library Preparation Kit using 1–2 μg of total RNA and were the...
Fast Start Universal SYBRgreen Master (Rox) (Roche, Mannheim, Germany) was used to perform RT-qPCR in accordance with the manufacturer’s instructions. Reactions were prepared in a total volume of 20 ml, comprising 10 ml of SYBR green master mix, 2 ml of cDNA template, 6 ml of...
TxshmceoelRltehhcosttdeemdrewfpraoosmrtmepdelabasnyutSrsaescdukbuejsteiacnltg.e6d6a, to water stress at the end of the drought treatment by a transient-pressure method, following the manufacturer's instructions. Scientific Reports | 6:20167 | DOI: 10.1038/srep20167 12 www.nature.com/...
Western blot. Cell lysates were prepared from motile sperms using RIPA buffer according to the manu- ®facturer's instructions (Beyotime, China). Fifty-six micrograms of denatured human sperm total proteins were separated on a 10% SDS-PAGE gel with a Criterion system (Bio-Rad, USA)...
Five of the Arabidopsis CCDs (NCED2, NCED3, NCED5, NCED6, and NCED9) catalyse the first step towards abscisic acid (ABA) biosynthesis [9]. The remaining four Arabidopsis members of this protein family (CCD1, CCD4, CCD7 and CCD8) have more divergent activities and catalyse the cleavage...
ABA: abscicic acid ACC: 1-aminocyclopropane-1-carboxylic-acid AHA: Aromatic, large Hydrophobic, Acidic context CST: complete sequence tree CTR: carboxy-terminal region ET: ethylene HALZ: homeodomain-associated leucine zipper HD-Zip: homeodomain-leucine zipper HSF: Heat Stress Transcription...
(KCNK3, NM_002246), TRAAK (KCNK4, AF247042), TWIK-1 (KCNK1, U33632), TASK-3 (KCNK9, AF212829), TREK-1b (KCNK2, AF004711) or TASK-4 (KCNK17, AF358910) cDNA were introduced with the QuickChange Site Directed Mutagenesis Kit (Stratagene) according to the instructions of ...
Semi-quantitative Real time analysis. Two μg of total RNA was used for the synthesis of cDNA using Superscript III cDNA synthesis kit (Invitrogen) according to the manufacturer's instructions. PCR was carried out in duplicates in a total volume of 10 μL reaction scale using Jump-...