Fitness Nerd (6) Galen Dara (10) Guest Post (7) Jaym Gates (8) Jenn E (2) John Ginsberg-Stevens (2) John Remy (2) JT Evans (9) Kate Sherrod (1) Mike Frighetto (2) Movies and Film (3) Music (5) Music Review (5) News (108) Paul Weimer (50) Podca...
Fitness Nerd (6) Galen Dara (10) Guest Post (7) Jaym Gates (8) Jenn E (2) John Ginsberg-Stevens (2) John Remy (2) JT Evans (9) Kate Sherrod (1) Mike Frighetto (2) Movies and Film (3) Music (5) Music Review (5) News (108) Paul Weimer (50) Podca...
FF Fitness Friend FF Frostfell (gaming, Asheron's Call) FF Final Frontier (gaming) FF Frontier Force FF Fuel Flow FF Frou Frou (band) FF Fit Factor FF Family Feud (TV game show) FF Feed Forward FF Fonky Family (French rap group) FF Fear Factor (TV show) FF Floor Flatness (concrete...
To identify putative doublets, we used the method implemented in the scDblFinder package [104] with default parameters. Barcodes identified as doublets had higher total UMI counts, number of detected genes, and were more likely to have more than one gRNA and were thus discarded. A total of ...
The CRISPR arrays were annotated with PilerCR [44] and the cas genes with CRISPRcasfinder [45]. The annotation plot was done with genoplotR [46] and the CRISPR repeat identity with the Weblogo online server [47]. Further the metagenomic spacers were extracted with a bash script (see scri...
Using CRISPRCasFinder [68], we identified CRISPR spacers targeting prophages of two “phage groups” in closely related hosts, validating the association of these prophages with their corresponding TM7 hosts. We identified CRISPR spacers and CRISPR-related proteins in genomes representing clades P1,...
Here we show that liver kinase B1 (LKB1) programs the metabolic and functional fitness of T reg cells in the control of immune tolerance and homeostasis. Mice with a T reg -specific deletion of LKB1 developed a fatal inflammatory disease characterized by excessive T H 2-type-dominant responses...
All 25 depleted synonymous RAD51C variants identified in the screen (10 slow-depleted, 15 fast-depleted) were subjected to ESE analysis using the ESE Finder 3.0 online tool: https://esefinder.ahc.umn.edu/cgi-bin/tools/ESE3/esefinder.cgi?process=home. The VaLiAnT generated ‘mseq’ (nucleotid...
Background Parasites are ubiquitously present across all of life [1,2]. Their negative impact on host fitness can impose strong selection on hosts to resist, tolerate, or escape potential parasites. Parasitoids are a special group of parasites whose successful reproduction is fatal to the host [...
gnfinderis a command line tool that finds scientific names from text using dictionary and nlp approaches. It serves as the engine of the online toolGlobal Names Recognition and Discoveryand is also used in many other platforms likeBiodiversity Heritage Libraryamong others. ...