The cells were lysed using 1× SDS-PAGE sample buffer containing 125 mM Tris (pH 6.8), 4% sodium dodecyl sulfate, 20% glycerol, 0.1% bromophenol blue, and 5% 2-mercaptoethanol, supplemented with 1× protease inhibitor (Complete EDTA-free tablet, Roche) and 1× phosphatase inhibitor (PhosSTO...
Samples were loaded onto 10 % SDS-PAGE gels (60 mM Tris, 0.1 % w/v SDS, 0.1 % w/v APS, 0.01 % v/v TEMED, 10 % Acrylamide/Bis (161–0156, Bio-Rad)) or 4-20 % Mini-PROTEAN® TGX Stain-Free™ gels (456– Minter et al. Acta Neuropathologica Communications (2016) 4:72...
Alternative splicing is an essential molecular mechanism that increase the protein diversity of a species to regulate important biological processes.Ecdysone receptor(EcR), an essential nuclear receptor, is essential in the molting, growth, development, reproduction, and regeneration of crustaceans. In th...