SDS: Sodium dodecyl sulfate PAGE: Polyacrylamide gel electrophoresis PVDF: Polyvinylidene difluoride TEMED: N,N,N′,N′-tetramethylenediamine Tris: 2-Amino-2-hydroxymethyl-1,3-propanediol TBST: Tris-buffered saline comprising Tween 2DE: Two-dimensional electrophoresis References Mair, A., Xu...
Crypt base columnar cells (CBCs) which are named because of their elongated/columnar appearance, reside at the bottom of the crypts, intermingled with the Paneth cells. CBCs are rapidly cycling and show characteristic expression ofLgr5[1]) andOlfm4[2]. The CBCs form rapidly proliferating trans...
(3) SDS-PAGE Gel preparation and running. Running gels: 15% acrylamide. Stacking gels: 6.1 mL of mQH2O, 2.5 mL of solution C (0.5 M Tris-HCl), 1.3 mL of solution A, 100 μL of solution D, 10 μL of TEMED and 50 μL of solution G. The samples had added loading buffer and ...
The resolving gel solution was composed of 8% 37.5:1 acrylamide:bis–acrylamide mixture, 350 mM Bis–Tris pH 6.8, 20 µM Phos-tag™ Acrylamide (Fujifilm Wako Chemicals Europe GmbH, Neuss, Germany), 40 µM ZnCl2, 0.1% tetramethylethylenediamine (TEMED), and 0.05% ammonium peroxysulfate ...
molecules Article Proteomics Analysis Reveals an Important Role for the PPAR Signaling Pathway in DBDCT-Induced Hepatotoxicity Mechanisms Yunlan Li 1,2,*, Xinxin Liu 1, Lin Niu 1 and Qingshan Li 1,2,* 1 School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, China; wei...