To create atpBA8D, the insert of petDA50 was isolated as an SstIHincII fragment, blunted with T4 DNA polymerase, and inserted into the BglII site of atpBA8, which had been filled in with the Klenow ...Stern DB, Jones H and Gruissem W (1989) Function of plastid mRNA 3′ inverted ...
coli RNA polymerase sigma factor sigma 70 in promoter-proximal pausing. Function of E. coli RNA Polymerase σ Factor- σ70 in Promoter-Proximal Pausing. Cell. 86: 485-493.Ring, B. Z., Yarnell, W. S., and Roberts,... BZ Ring,WS Yarnell,JW Roberts - 《Cell》 被引量: 258发表: ...
Direct activation of RNA polymerase III transcription by c-Myc. Nature. 2003;421:290–4. Article CAS PubMed Google Scholar Felton-Edkins ZA, Fairley JA, Graham EL, Johnston IM, White RJ, Scott PH. The mitogen-activated protein (MAP) kinase ERK induces tRNA synthesis by phosphorylating ...
The influenza virus-associated RNA polymerase cleaves capped RNA in an endonucleolytic manner and the transcription is initiated by the addition of GMP, the first substrate to be polymerized under the direction of viral RNA template, onto 3'-termini of resulting capped RNA fragments. In the prese...
Structure and membrane topography of the Vibrio-type secretin complex from the type 2 secretion system of enteropathogenic Escherichia coli. J. Bacteriol. 200, e00521-17 (2018). Google Scholar Fei, J. & Sharma, C. M. RNA localization in bacteria. Microbiol. Spectr. https://doi.org/...
The standard H-type folding topology shown is nearly exclusively observed in natural RNA sequence contexts. An RNA pseudoknot was first recognized as a novel RNA folding motif by Rietveld, Pleij and coworkers in transfer RNA-like structures found at the 3′ end of turnip yellow mosaic virus (...
int, print progress information for every number of samples completed, default: 100. cd LucaProt/src/prediction/ sh run_predict_many_samples.sh Or: cd LucaProt/src/ # using GPU(cuda=0) export CUDA_VISIBLE_DEVICES="0,1,2,3" python predict_many_samples.py \ --fasta_file ../data/rdrp...
Using the sequence data obtained from the CαCδ region, we have designed simple assays to test for Jα gene segment transcription and to determine the level of polymorphism for simple repeat sequences among different inbred strains of mice using the polymerase chain reaction. Furthermore, ...
coli RNA polymerase alpha subunit (PDB code 4JK1), showing similarity in parts of their structures. b, Possible arrangement of cyanophycin that allows either its positive charges or negative charges to interact with αa or αb. c, Activity assays of TmCphA1 N domain mutants showing similar...
Next, real time polymerase chain reaction (RT-qPCR) was conducted with the QuantiNova SYBR Green PCR Kit (QIAGEN, Product of Germany). Lastly, β-Actin was employed for normalization of gene expression. M6A Immunoprecipitation (MeRIP) 1–3 μg of total RNA and m6A spike-in control were ...