RNA-binding proteins (RBPs) play an essential role in regulating the function of RNAs in a cellular context, but our ability to control RBP activity in time and space is limited. Here, we describe the engineering of LicV, a photoswitchable RBP that binds to a specific RNA sequence in resp...
An important regulator of mRNA decay is the RNA binding protein CUGBP1 and Etr3 Like Factor 1 (CELF1). CELF1 binds to a GU-rich element (GRE) harbored in target transcripts and promotes their rapid decay through the recruitment of RNA decay enzymes. The work presented in this thesis ...
(CSD). A small (∼70 kDa) domain with high similarity to the ribonucleoprotein 1 RNA-binding motif. This domain is found in DNA- and RNA-binding proteins in bacteria, archaea and eukaryotes. Interfaces The solvent-accessible portions of a protein that are capable of binding a ligand — in...
when scientists at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, set out to find enzymes - the proteins that carry out chemical reactions inside cells - that bind to RNA, they too found more than they expected: 300 proteins previous...
RNA-binding proteins (RBPs) play an essential role in regulating the function of RNAs in a cellular context, but our ability to control RBP activity in time and space is limited. Here, we describe the engineering of LicV, a photoswitchable RBP that binds to a specific RNA sequence in resp...
Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. H
How does the structure of RNA differ from the structure of DNA? Functions of RNA in Protein Synthesis Cells access the information stored in DNA by creating RNA to direct the synthesis of proteins through the process oftranslation. Proteins within a cell have many functions, including building ce...
Here, they modulate the specific binding of proteins to their cognate mRNAs, thereby regulating or autoregulating the expression of the downstream gene. RNA pseudoknots can also be present within the coding regions of mRNAs, where they stimulate programmed −1 ribosomal frameshifting, an essential ...
Perhaps the most striking example of RNA structure being critical to an essential cellular process is the ribonucleoprotein complex that constitutes the translational machinery. Ribosomal RNA (rRNA) molecules act as a scaffold on which proteins are assembled to form...
RBPs RNA-binding proteins MREs miRNA response elements CDR1as cerebellar degeneration-related protein 1 antisense 3′-UTR 3′-untranslated region ciRS-7 circular RNA sponge for miR-7 AKT3 AKT serine/threonine kinase 3 CDK2 cyclin-dependent kinase 2 ID1 anti-senescence protein inhibitor of different...