The PCR three main phases which are Denaturation, Annealing and Elongation. During Denaturation, the high temperature is provided to break the hydrogen bonds between the two DNA strands and separate them. Annealing is the phase where the forward and reverse primers attach to the DNA str...
Strikingly, EZH2 deficiency impairs the translation process globally and reduces internal ribosome entry site (IRES)-dependent translation initiation in cancer cells. Our findings reveal a previously unrecognized role of EZH2 in cancer-related translational regulation....
The sequences of the primers used in this study are shown in Additional file 7: Table S6. H&E staining of tissues Mouse organ tissues were fixed with 4% PFA (Servicebio, Wuhan, China) and dehydrated using a graded ethanol series, followed by clearing with xylene (Shanghai, China). The...
tin1, within a 3.9-kb fragment on maize chromosome 7. We show thattin1encodes a C2H2-zinc-finger transcription factor. A splice-site variant from G/GT to C/GT in sweet/popcorn leads to intron retention, then may enhance mRNA stability and transcription oftin1, and...
microPrimer: the biogenesis and function of microRNA. (2005) microPrimer: the biogenesis and function of microRNA. Development, 132, 4645-4652.Du, T., and Zamore, P.D. (2005). microPrimer: the ... P Team 被引量: 0发表: 0年 Real-time PCR quantification of precursor and mature micro...
Sequence Determination of A. thaliana Cryptochrome DASH We determined the Arabidopsis cDNA sequence corresponding to the entire Synechocystis cryptochrome gene which was amplified from RNA (Col-O) by PCR with the following primers: 5′-ATGAACGACCATATCCACCG-3′ and 5′-GATACAAGTAGTTCGTGTAAAGA-...
The fimA genotype was determined either by PCR using genotype-specific primers or from the presence or absence of restriction enzyme cleavage. The fimA genotypes I, II, III, IV, and V have been classified and are associated with P. gingivalis virulence [11,34,35]. By contrast, mfa1 may ...
The promoter region of NFL1 gene in tobacco was cloned by a novel PCR method,fused with the report gene GUS and transformed tobacco plants.The expression of GUS in the shoot apex of transgenic seedlings was detected.关键词: tobacco NFL gene promoter 被...
The sequences of the primers are listed. The relative expression of target genes was normalized to the expression of the gene encoding mHprt or hGAPDH. The relative mRNA expression of other genes was measured using the standard 2(−△△CT) method. The sequences of primers were as ...
Postoperative cognitive dysfunction (POCD) affects the outcome of millions of patients each year. Aging is a risk factor for POCD. Here, we showed that surgery induced learning and memory dysfunction in adult mice. Transplantation of feces from surgery m