Its unusually high concentration and rapid turnover in brain make it accessible to both proton and C NMR spectroscopy methods. This has enabled researchers to study the various functions of glutamate in basic neuroscience and brain disorders noninvasively and in vivo. Here, we provide an overview ...
Recent advances in the application of solution NMR spectroscopy to multi-span integral membrane proteins Integral membrane proteins (IMPs), which can only be released from the membrane by disruption of the membrane, perform a host of vital cellular functions a... HJ Kim,SC Howell,WDV Horn,.....
NMR spectroscopy has become an indispensable tool for high-resolution structure determination of biomolecules at physiological conditions both in solutions and solids. Currently, NMR is routinely...doi:10.1007/s41745-018-0085-1Rai UpasanaSharma Rakhi...
the more likely molecular mechanisms of proteins function could be revealed accurately.In-cell study of protein structure and function has been a frontier topic in protein science.Nuclear magnetic resonance(NMR)spectroscopy is the most promising technique for protein structural and functional assay at ...
In this study, we interrogated the urinary metabolome using nuclear magnetic resonance (NMR) spectroscopy to compare individuals with known low and high DON exposure through consumption of their normal diet. Urine samples from 22 adults from the UK (seven males, 15 females; age range = 21–59 ...
R. Elucidating binding mechanisms and dynamics of intrinsically disordered protein complexes using NMR spectroscopy. Curr. Opin. Struct. Biol. 54, 10–18 (2019). Article CAS PubMed Google Scholar Teilum, K., Olsen, J. G. & Kragelund, B. B. On the specificity of protein–protein ...
We expressed the EL5 RING-H2 finger domain in Escherichia coliand determined its structure in solution by NMR spectroscopy. The EL5 RING-H2 finger domain consists of two-stranded β-sheets (β1, Ala147–Phe149; β2, Gly156–His158), one α-helix (Cys161–Leu166), and two large N- ...
Multi-dimensional NMR spectroscopy was used to characterize the solution structural and dynamic properties of the v-Myc bHLHZip domain. Significant secondary chemical shifts indicate the presence of two separated α-helical regions. The C-terminal leucine zipper region forms a compact α-helix, while...
The dual-function NMR probe is useful in continuous flow-through, stop-flow, and fixed-sample analysis applications, including applications that couple high-pressure liquid chromatography (HPLC) with nuclear magnetic resonance spectroscopy and applications for analyzing small quantities of stationary organic...
nmrproteinspectroscopymechanisticcluespathogenesis 8NMRSpectroscopyasaTooltoProvideMechanisticCluesAboutProteinFunctionandDiseasePathogenesisBenjaminBourgeois1,HowardJ.Worman2andSophieZinn-Justin11LaboratoiredeBiologieStructuraleetRadiobiologie,CEASaclay&URACNRS2096,Gif-sur-Yvette2DepartmentofMedicineandDepartmentofPathologyand...