Recently, utilization of Machine Learning (ML) has led to astonishing progress in computational protein design, bringing into reach the targeted engineering of proteins for industrial and biomedical applications. However, the design of proteins for emerg
Adipose-derived stem cells (ADSCs) have been extensively used in preclinical and clinical trials for treating various diseases. However, the differences between ADSCs from lean individuals (L-ADSCs) and those from obese individuals (O-ADSCs) have not bee
Cell pellets were lysed with lysis buffer (20 mM Tris, 150 mM NaCl, 5 mM MgC12, 1 mM DTT, 25 U/mL DNaseI, 100 μg/mL cycloheximide, 1% (V/V) TritonX-100, 0.1% (V/V) NP-40) for 10 min on ice, followed by centrifugation at 13,000 × g for 10 min at ...
Diabetes mellitus (DM)-related complications are important health problems worldwide. The underlying mechanisms for diabetic male subfertility/infertility are considerably complicated and need to be unveiled for therapeutic intervention. Melatonin treatm
Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. H
The reason is the structure of p53 protein folding dynamic change and intrinsically disordered domain [3, 4]. Studies have shown that most p53 mutations have missense mutation preferentially occurred in the DNA binding domain, as a result of single amino acid change at many different positions [...
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By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection. See our privacy policy for more information on the use of your perso...
Despite the absolute processivity of cellular RNAPs, transcript elongation is far from a smooth, continuous process. Due to specific regulatory signals or nonspecific obstacles to the forward progress of the enzyme, elongating RNAP frequently pauses at sites on the DNA template. In addition to trans...
The cells were then lysed on ice in SDS lysis buffer (50 mm Tris-HCl, pH8.0, 10 mm EDTA, 150 mm NaCl, 1% SDS) supplemented with 1× proteinase inhibitor mixture (Roche Applied Science). Then the lysate was sonicated on ice (10-s pulse, 50-s break, six times, 20% amplitude; ...