英文:The researchers optimized the composition of the lysis buffer to improve the efficiency of protein extraction. 英文同义表达: cell lysis buffer:特指用于裂解细胞的缓冲液。 说明:这种表达更具体地指出了缓冲液用于裂解细胞的目的。 extraction buffer:在提取生物分子时使用的缓冲液...
For modified lysis buffer, we researched the effects of pH and composition of reagent. The addition of PVP, DMSO and EDTA.Na2 has been proved to reduce the inhibitory during the extraction of DNA from Conclusion In this study, a Chitosan@Beads-based DNA extraction method was established. The...
Composition 25 mM Tris-HCl, pH 7.6 150 mM NaCl 1% NP-40 1% sodium deoxycholate 0.1% SDS A modified RIPA buffer formulation without SDS Non-denaturing detergent in 25 mM bicine buffer (pH 7.6) 50 mM Tris, pH 7.4 250 mM NaCl 5 mM EDTA...
Methods: The alkaline lysis buffer was used for rapid extraction of DNA from traditional Chinese medicines(TCM) and the effects of the extracting solution's formula composition and types\nof neutralizing reagents on the purity and concentration of Chinese medicines DNA were investigated.DNA of 144 ...
Composition 25 mM Tris-HCl, pH 7.6 150 mM NaCl 1% NP-40 1% sodium deoxycholate 0.1% SDS A modified RIPA buffer formulation without SDS Non-denaturing detergent in 25 mM bicine buffer (pH 7.6) 50 mM Tris, pH 7.4 250 mM NaCl 5 mM EDTA...
The mixture after the cell lysis procedures is referred to aslysate; a “bimolecular soup” of the cell content. The buffer content, the choice ofenzyme, and the approach to lysis differ according to the organism, material, or nucleic acid of interest. ...
Ignatoski KMW, Verderame MF: Lysis buffer composition dramatically affects extraction of phosphotyrosine-containing proteins. BioTechniques 1996, 20: 794–796. CAS PubMed Google Scholar Berkelman T, Brubacher MG, Chang H: Important factors influencing protein solubility for 2-D electrophoresis. BioRa...
Ignatoski KMW, Verderame MF: Lysis buffer composition dramatically affects extraction of phosphotyrosine-containing proteins. BioTechniques 1996, 20: 794–796. CAS PubMed Google Scholar Berkelman T, Brubacher MG, Chang H: Important factors influencing protein solubility for 2-D electrophoresis. BioRa...
assaysMild,non-denaturingand efficient lysis forcytoplasmic and nuclearprotein extractionRecommended for extraction ofcytoplasmicproteinsNeed a harsher buffer than NP-40 or whencytoplasmic and nuclearprotein extraction is needed Composition25 mM Tris-HCl, pH 7.6 ...
assaysMild,non-denaturingand efficient lysis forcytoplasmic and nuclearprotein extractionRecommended for extraction ofcytoplasmicproteinsNeed a harsher buffer than NP-40 or whencytoplasmic and nuclearprotein extraction is needed Composition25 mM Tris-HCl, p...