The instrument operated in Full MS-ddMS2 mode for simultaneous MS1 and MS/MS acquisition, with the following parameters: sheath gas pressure, 30 arb; auxiliary gas flow, 10 arb; spray voltages, 3.50 kV and −2.50 kV for ESI( +) and ESI(-), respectively; capillary temperature, 325 ...
Full MS followed by data-dependent MS–MS (ddMS2) spectra were obtained at 35,000 resolution (200 m/z) with a scan range of 50–750 m/z and a stepped normalized collision energy corresponding to 5, 11, and 17 eV. The injection volume was 10 µL and samples were maintained at 4 ...
(2) UPLC-MS analysis. The equipment used in the analysis included the CBM-20 A system controller, LC-20ADXRpump, SIL-20ACXRautosampler, CTO-10Avp column box and equipped with protective columns (C18, 4 mm × 3.0 mm, Phenomenex Co., Ltd., Torrance, CA, United States) and Shiseido C18...
BC breast cancer, TNBC triple-negative breast cancer, MSP methylation-specific PCR, FFPE formalin-fixed paraffin-embedded, ddMSP, droplet digital methylation-specific PCR Full size image Table 2 Epigenetic markers employed in the ddMSP assay Full size table Performance of the internal control marker...
Prior to data-dependent MS3 (ddMS3) analysis, RTS was utilized with the UniProtKB Mus musculus (sp_tr_incl_isoforms TaxID = 10,090) protein database including cysteine carbamidomethylation (+ 57.0215 Da), TMTpro16plex (+ 304.20171 on Kn), and methionine oxidation (+ 15.9949)....
2 μL, respectively. Mass spectrometric detection of metabolites was performed on Q Exactive Focus (Thermo Fisher Scientific, USA) with an ESI ion source. Simultaneous MS1 and MS/MS (Full MS-ddMS2 mode, data-dependent MS/MS) acquisition was used. The positive and negative ion modes were ...
A voucher specimen (DDMS 4598) was deposited at the Herbarium of the Federal University of Grande Dourados, Dourados, MS, Brazil. Extraction, fractionation and isolation procedure The air-dried and powdered aerial parts of A. dioica (850 g) were successively extracted via maceration with methanol...
energies Article Design Consideration of Bidirectional Wireless Power Transfer and Full-Duplex Communication System via a Shared Inductive Channel Jie Wu 1,*, Weihao Kong 1 , Pengfei Gao 1, Nan Jin 1 , Jitao Zhang 1, Jiagui Tao 2 and Václav Snášel 3,* 1 School of Electrical and ...
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Für KEEP 2018 endete die Haltefrist zum 2. Oktober 2021 und die kostenlosen Bonus-Aktien wurden an die berechtigten Mit- arbeiter ausgegeben. Im Geschäftsjahr 2021 wurde im Rahmen des Mitarbeiteraktienoptionsprogramms für Gratis-Aktien und Bonus-Aktien ein Aufwand in ...