A variety of widely targeted metabolomics strategies have been published, such as the full scan and ddMS2 mode combination method [39], SWATH-MS method [40], and multiple ion monitoring enhanced product ion method (MIM-EPI) [41]. We obtained all the information on non-targeted metabolomics ...
Full MS followed by data-dependent MS–MS (ddMS2) spectra were obtained at 35,000 resolution (200 m/z) with a scan range of 50–750 m/z and a stepped normalized collision energy corresponding to 5, 11, and 17 eV. The injection volume was 10 µL and samples were maintained at 4 ...
CDK1 is upregulated in stem-cell enriched epidermal cultures. (a) Scheme describing the experimental pipeline for mass spectrometry analysis; abbreviations: EPSC = Epidermal Stem Cell, TAC = Transient Amplifying Cell, TD = Terminally Differentiated cell, LC-MS = Liquid Chromatograph...
Prior to data-dependent MS3 (ddMS3) analysis, RTS was utilized with the UniProtKB Mus musculus (sp_tr_incl_isoforms TaxID = 10,090) protein database including cysteine carbamidomethylation (+ 57.0215 Da), TMTpro16plex (+ 304.20171 on Kn), and methionine oxidation (+ 15.9949)....
The chemical components of CMF were identified using the UFLC-Q-TOF-MS/MS combined system. Data mining and the wSDTNBI algorithm were combined to construct a differential expression gene (DEG) - CMF prediction target interaction network for CRA. The core targets of CMF in CRA prevention and ...
ddMSP: droplet digital methylation-specific PCR ELMOD3 : ELMO/CED-12 domain containing 3 FFPE: formalin-fixed paraffin-embedded GLYATL3 : glycine N-acyltransferase-like protein 3 HER2: human epidermal growth factor receptor 2 HOXA10 : homeobox A10 HV: healthy volunteer JT test: Jonckh...
A voucher specimen (DDMS 4598) was deposited at the Herbarium of the Federal University of Grande Dourados, Dourados, MS, Brazil. Extraction, fractionation and isolation procedure The air-dried and powdered aerial parts of A. dioica (850 g) were successively extracted via maceration with methanol...
Inhibition of the CYP450-4A enzyme using DDMS blocked vasoconstriction to an increase in P O2, but had no effect on dilation to low P O2. Conclusions We conclude that: 1) L-type Ca2+ channels are central to oxygen sensing, 2) KATP channels are permissive for the arteriolar response to ...
energies Article Design Consideration of Bidirectional Wireless Power Transfer and Full-Duplex Communication System via a Shared Inductive Channel Jie Wu 1,*, Weihao Kong 1 , Pengfei Gao 1, Nan Jin 1 , Jitao Zhang 1, Jiagui Tao 2 and Václav Snášel 3,* 1 School of Electrical and ...
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