Therefore, using whole-exome and whole-genome sequencing (WES and WGS, respectively), we aimed to identify novel PAVs and protein-truncating variants (PTVs, as putative loss-of-function variants) affecting serum lipid and lipoprotein measurements, complemented with serum nuclear magnetic resonance (...
In this study, we combined EQUC of urine obtained primarily from adult females by transurethral catheterization with large-scale whole-genome sequencing to generate a comprehensive bladder-specific bacterial isolate genome reference collection. To assess the completeness of this catalog, we compared the ...
This price includes RT and PCR amplification, as well as final library preparation according to recommendations by manufacturer (Fig. 2B). From here on, this protocol is referred to as Takara®. G&T Protocol Genome & Transcriptome sequencing (G&T-seq) is not a commercially available protocol, ...
To assemble the genome ofC. camphora, a combination of Illumina short-read sequencing and PacBio long-read sequencing technology was applied. The sequencing ofC. camphoraresulted in coverage of ~161.65-fold PacBio single-molecule long reads (111.06 Gb with an average length of 18,420 bp), and...
The capacity of NGS has steadily increased and with the latest generation of sequencing platforms, an entire human genome can be sequenced within 2 days at the price of a few hundred dollars. The relatively modest costs per analysis, combined with excellent data quality [10], make whole genome...
Metagenome sequencing and assembly Metagenomes were sequenced at the Max Planck Genome Centre Cologne, 34 on an Illumina HiSeq 2500 using 2 × 150 bp chemistry, and eight additional PA3 metagenomes on a PacBio Sequel II (Menlo Park, CA, USA) using one SMRT cell per sample in long-read ...
the rapid adoption of genome sequencing has been fueled by rapid dropping of cost, and this may be the main determining factor in which platforms are used for any given clinical trial. The commercial price of a whole genome sequencing declined from more than $50,000 in 2009 to less than $...
Intra-tumour heterogeneity (ITH) presents a significant obstacle in formulating effective treatment strategies in clinical practice. Single-cell RNA sequencing (scRNA-seq) has evolved as a powerful instrument for probing ITH at the transcriptional level,
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