We generated ex vivo tumor slice cultures from fresh surgical resections of gastric and colon cancer and treated them with GITR agonist or TIGIT antagonist antibodies. We applied paired single-cell RNA and TCR sequencing to the original surgical resections, control, and treated ex vivo tumor slice...
2g). Similarly, full-length coverage with Smart-seq3xpress resulted in significantly (2–5-fold) increased read support over exon–exon and exon–intron splice junctions, which form the basis for RNA velocity inference16 (Fig. 2h). To perform a direct comparison of cluster granularity, we ...
CAR structure and generations. CAR T cells are composed of three functional regions from the inside out: (1) the signaling domain of the TCR, (2) the transmembrane domain, and (3) the scFv. 1st generation: CD3ζ as signal transduction domain;2nd generation: additional costimulatory signaling ...
CD4+T lymphocytes dominate the Th cell population and the T cell receptors (TCR) in Th cells are involved in the recognition of antigens. Th1 cells are differentiated under the direction of IL-12 and mainly secrete interleukin-2 (IL-2), interferon-γ (IFN-γ), and tumour necrosis factor-...
Instructions for accessing Generation Scotland data can be found here: https://www.ed.ac.uk/generation-scotland/for-researchers/access; the ‘GS Access Request Form’ can be downloaded from this site. Completed request forms must be sent to access@generationscotland.org to be approved by the ...
CAR-T cell therapy is an innovative form of cancer immunotherapy that involves modifying a patient's own T cells to enhance their ability to recognize and attack cancer cells [21]. The process begins by collecting the patient's T cells from their blood. These T cells are then genetically en...
(e.g., distinct cell-cell contacts of cardiomyocytes, intercalated discs (ICD) [3]). While cardiomyocytes make up most of the heart by volume, they are outnumbered by a diverse mix of fibroblasts, immune cells, endothelial cells, and mural cells, which form the organ scaffold and vascular ...
T-cell receptor (TCR)-based adoptive therapy employs genetically modified lymphocytes that are directed against specific tumor markers. This therapeutic modality requires a structured and integrated process that involves patient screening (e.g., for HLA-
In the process of Agrobacterium-mediated genetic transformation, hypocotyls from Jin668 can undergo dedifferentiation and re-differentiation to form embryogenic callus and finally regenerate plants via somatic embryogenesis (Fig. 1a), while TM-1 can only produce non-embryogenic callus that cannot undergo...
Considering the limitations of CAR-T, some new ideas for ACTs have been emerging. One is an alternative cell of origin to construct armed autologous or allogenous immune cells with the CARs; the other is to screen and identify HLA-restricted tumor-specific targeting-TCR and then build genetical...