Jin-Gui-Shen-Qi Wan (JGSQ) has been used in China for thousands of years to treat various ailments, including frequent urination, blurred vision, and soreness in the waist and knees. It has traditional therapeutic advantages in improving eye diseases. Cl
Prediction of RNA secondary structure RNA secondary structures were analyzed by the Mfold program [44]. The structure drawings were edited and annotated using the RnaVis 2 program [45]. SDS-PAGE, Western blot analysis, and MALDI-TOF analysis For Western blot analysis, protein samples were resolve...
Aβ peptides are classified into two major isoforms composed of 40 or 42 amino acids (Aβ40 and Aβ42), with Aβ42 being the more toxic form which plays an essential role in the pathogenesis of AD. We investigated whether YXT and its herbal components could inhibit the Aβ42 ...
natriegens Vmax could form soluble and active MsAA upon induction with 1 mM IPTG. Therefore, we conducted the chaperone co-expression experiments at 30 °C cultivation temperature for 4 h. Fig. 3 SDS-PAGE analysis of cell extracts obtained from V. natriegens Vmax™ after expression of ...
The activation of caspase-3 in DHA-treated ASTC-a-1 cells was examined by Western blotting which visualized the processing of the inactive caspase proform to the catalytically active, smaller units. For Western blot analysis, the cells were treated with 1 μM STS for 12 h, 20 μg/m1 DHA...
For docking of the natural substrate of 4-aminobutyrate aminotransferase, the substrate molecules were separately saved from a known structure (PDB ID: 4ATQ) [49] as a single molecule in the form of an external aldimine and used as a ligand. For docking with 5AVA aminotransferase, pyridoxal ...
5, lane 5) if pGC-A protein did not form crystals and stayed in solution. Additionally, in Fig. 5, lanes 3 and 5 showed strong pGC-A signals above the 250 kDa molecular weight in the western blot. Nevertheless, the main protein signal band in the sample of the purified protein before...
First, through microarray analyses of the AU-rich transcriptome in pituitary αT3-1 cells, we identified 34 ARE-containing transcripts upregulated in cells expressing a constitutively active form of AKT1. In parallel, by an affinity chromatography-based technique followed by microarray analyses, 12 ...
In vitro functional analysis of miR-188-3p supported the tumor suppressing role in breast cancer cells, taking the form of suppression of proliferation, migration and invasion in MCF-7 cells. As further verification, Pearson correlation analysis on tissue level corroborated the luciferase reporter ...
Next, to validate whether the downregulated YAP1 rewires stromal cells for enhanced production of stimulatory growth factors such as GROα to form a premetastatic niche in vivo, stromal-specific Yap1 conditional knockout (cKO) mice bearing two loxP sites flanking the exons of the Yap1 gene were...