A fundamental tenet of the hygiene theory is the inverse association between helminth infections and the emergence of immune-mediated diseases. Research has been done to clarify the processes by which helminth-derived molecules can inhibit immunological
The full-length mouse Engrailed-2 (En2) sequence was amplified from cerebellar mouse tissue via PCR and subsequently cloned into a pQE80L vector containing HIS/GST tag sequences. The EN2 protein was then produced in BL-21 bacteria and purified by passage over a GST affinity column. Following ...
The Eco RI+Bam HI region of PQE60 was inserted in pQE80L vector where Xho I, Nco I and Mfe I sites have been mutated. Then the LacZ encoding gene was PCR amplified using primers containing flanking sequences (represented in bold) used for LIC cloning: 5'-CCATGG CTcatcaccatcaccatcacGG...
However, significant improvements have been achieved by protein engineering of the β-carotene ketolase. By using random mutagenesis, crtW mutants form Paracoccus sp. and Sphingomonas sp., were generated that showed an up to 81% [22] and 90% [23] production of astaxanthin, respectively, when ...
The E. coli (pQE-adhE2/ptb/buk) strain produced 1.7 mM butanol from 2.3 mM butyric acid after 120 h of incubation under anaerobic condition. Interestingly, we observed that butyrate concentration in the E. coli (pQE – ptb/buk) strain increased when grown for 48 and 120 hours, leading ...
The gene choA encoding a cholesterol oxidase from Chryseobacterium gleum DSM 16776 was cloned into the pQE-30 expression vector and heterologously expressed in Escherichia coli JM109 co-transformed with pRARE2. The N-terminally His-tagged cholesterol oxidase (CgChoA) was assigned to be a monomer ...
Production and purification of enzymes Expression strain E. coli M15[pREP4] harbouring plasmid derivatives of pQE-30-LE encoding for single or fusion genes were grown in high-cell density fermentation and crude extracts were prepared for protein isolation. Two purification steps using double-tag pro...
Venuti, using specific primers carrying restriction sites useful for cloning in pQE-30 expression vector (QIAGEN), and were expressed as MRGS (H)6 tag proteins (Table 1). Cloning and expression of E7 (297 nt) were previously described by Accardi et al. 2005. A truncated form of L1 gene...
IP ≈ 4A (68) Estimated FET Vds rise and fall time: tr ≈ tf = 100nC − IP 2 52nC = 48nC 4A 2 ≈ 24 ns (69) Estimate QE and QF FET Losses (PQE): PQE = IQE_RMS2 × Rds on QE + POUT VOUT × VdsQE tr + tf fs 2 + 2 × COSS_QE_AVG × VdsQE2 fs 2 + 2 ...
IP » 4 A (84) Estimated FET Vds rise and fall time using Equation 85: t r » t f = 100nC - 52nC I = 48nC 4A » 24 ns P 22 (85) Estimate QE and QF FET Losses (PQE) using Equation 86: PQE I u R2 QE _RMS ds(on)QE POUT VOUT u VdsQE tr tf fSW 2 u COSS_...