The primer sequences used to amplify the internal regions of the basic endochitinase Ppbec1 (BEC226F and BEC576R), dehydrin Ppxero2 (DX-82F and DX176R), thaumatin Pptha1 (THA30F and THA382R), lipoxygenase Pplox1 (LOX982F and LOX1267R) and the actin Ppact7 (ACT-F and ACT-R) ...
the protein, while removal of the first 25 amino acids from the N-terminus led to dominant negative cell cycle arrest that could be reversed by simultaneous overproduction of Cdc27, suggesting that the mutant Cdc1-NΔ2–25 protein exerted its function by titrating Cdc27 from the cell [20]....
The data in this paper suggest that the signal peptidase I recognition site is protected in the membrane bound form of P80, but is accessible as the first step in the release of the helical part of P80 into the cell culture supernatant, a process which is accompanied by a decrease in size...