The biosynthesis of monoterpene derived from NPP has demonstrated an increase in monoterpene production in S. cerevisiae and Y. lipolytica [12, 19]. Furthermore, enzyme fusion has been investigated as a strategy to facilitate the conversion of precursors into terpenes [1, 6]. Thus, we expressed...
Structure and localization of 5-HT6 receptors Initially cloned from striatal tissue [5], the rat 5-HT6 receptor gene encodes a protein of 438 amino acids and shares 89% homology with the human form [6, 7]. The 5-HT6 receptor belongs to the G-protein-coupled receptor (GPCR) family, ...
Nanotechnology has brought a variety of new possibilities into biological discovery and clinical practice. In particular, nano-scaled carriers have revolutionalized drug delivery, allowing for therapeutic agents to be selectively targeted on an organ, ti
(reward wanting, often compared to the human experience of craving) and measures the amount of work or effort that a subject is willing to put in to obtain a reward, in this case rewarding food in the form of sucrose, and it therefore mainly measures the wanting component of food reward....
MPH-induced dynamics in DMN connectivity patterns with other neural networks may account for some of the effects of MPH administration on impulsive behavior.Introduction The human brain is functionally organized into distinct regions that collaboratively form extensive, interconnected neural networks [1]. ...
(Beyotime). To quantify alkaline phosphatase activity, 500 µL of p-NPP solution was added and incubated in dark for no more than 3 min. The cells were measured by reading the absorbance at 405 nm in triplicate using multifunctional enzyme marker. After 21 days of osteogenic culture, cells...
and further isomerizes to the cis-LPP. The cis-LPP undergoes the second ionization, generating the neryl cation, which can lead to acyclic monoterpenoids by acyclic MTPSs including nerol synthase (NerS) [37]. Notably, the cisoid substrate NPP can be ionized directly to form the neryl ...
Formation of glucosylceramide is particularly important because of its role in conferring drug resistance to tumor cells [29]. In addition, ceramide can be directly phosphorylated by ceramide kinase (CerK) to form C1P (Fig. 1), which is a key regulator of cell homeostasis [18, 30] and has...
most severe form of FASD, is estimated to be > 100,000 per year [11]. Whilst the effects of PAE on craniofacial differences are well documented [12,13,14,15], studies investigating the clinical effects on the rest of the skeleton are limited. Early small-scale studies and case reports...
To investigate the activity of the recombinant RM lipase, p-NPP was used as the substrate. In a standard assay, the reaction mixture contained 40 µL of p-NPP (12.5 mM), 940 µL of Tris-HCl buffer (50 mM, pH 8.0) and 20 µL of diluted enzyme solution. The mixture was incuba...