Function annotation and differential analyses of gut microbiota dysbiosis.AFunctional implications of gut microbial dysfunction in patients with CAD; The x axis indicates the log2-transformed fold change of each functional annotation.Pvalue for each functional annotation was showed in the plot. The func...
The vaginal microbiota guards the entry of the reproductive tract. In concert with immune cells and the vaginal mucosa, it provides a physical and (bio-)chemical barrier against pathogens, preventing gynecological infections. A healthy vaginal microbiome is dominated by non-pathogenicLactobacillusspp.,p...
Hypervariable microbial 16s rRNA regions V3 and V4 were identified by PCR using the primers (341F: CCTACGGGNGGCWGCAG and 805R: GACTACHVGGGTATCTAATCC, taken from the manual “16S Metagenomic Sequencing Library Preparation”, Illumina, San Diego US). The Microbial Genomics Module of Qiagen CLC...
Analysis of the 16S rDNA V3-V4 regions from nudibranch body organs The same samples used for the nested PCR described above were used for amplification of the V3 and V4 regions of the 16S rDNA using primers 341F: 5′-GCT CTT CCG ATCT -N- CCT ACG GGN GGC WGC AG-3′ and 805R: 5...
of terminal complementarity, retaining in the mature form only four to five nucleotides upstream of the c box and two to five nucleotides downstream of the d box. sequencing of the small rna population as well as of the small rnas isolated after ago2 immunoprecipitation revealed that despite ...
(e.g., GGN for conserved glycines or CCN for conserved prolines) can be inferred to have been present already in the common ancestor of all extant eukaryotes, so the ancient protosplice sites (if such existed) should have survived and could be examined directly. This analysis has shown ...
Redundancy analysis (RDA), a form of constrained ordination, was employed to analyze the relationships between the abundance of core genera and environmental variables using R software with the vegan and ggplot2 packages. Results Biodiversity and microbial community profiles in Carrousel system Using ...
Amplicon libraries were constructed according to the protocol from Illumina (16S Metagenomic Sequencing Library Preparation) that amplified the V3-V4 region of the bacterial 16S rRNA gene: 341F (5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG- 3′) and 805R (5′-GTCTCGTGGGCTCGGAGATG...
Besides rhizobia, legume plants also recruit different subsets of microbial communities depending on their ability to form nodules or not [18, 19]. The design of microbial inoculants that can lead to increased crop production and/or resilience to climate stresses is a promising research direction....
NmeCas9 and several other type II-C Cas9 orthologs have been shown to possess an RNA-dependent ssDNA cleavage (DNase H) activity in vitro [52,55]. R-loops (regions where an RNA strand invades a DNA duplex to form a DNA:RNA hybrid, with the other DNA strand displaced) occur naturally ...