Based on synteny analysis, both the location and orientation of LAT and its adjacent genes, including SH2B1, ATXN2L, SPNS1, NFATC2IP, and SGF29, were evolutionarily conserved among the teleost species (Figure 1A). In addition to LAT, other components that form the signalosome in mammals, ...
suspensions of the spleens and lymph nodes were prepared, and the frequencies of splenocytes labeled with high and low concentrations of CFSE were analyzed by flow cytometry. The specific lysis percentage was calculated according to the published formula [22]: %specificlysis=CFSElow×α−CFSEhi...
Single-cell chromatin accessibility has emerged as a powerful means of understanding the epigenetic landscape of diverse tissues and cell types, but profiling cells from many independent specimens is challenging and costly. Here we describe a novel approach, sciPlex-ATAC-seq, which uses unmodified DNA...
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Cells were stained with CFSE and cultured for 48–72 h before measuring the intensity of fluorescence to detect cell division (Beyotime). For the apoptosis assay, the cells were stained using the APC Annexin V Apoptosis Detection Kit with Propidium Iodide (PI) (Biolegend) according to the ...
An mPEG -PCL nanoparticle was used for encapsulation of an IDO1i (epacadostat) and an HDACi (panobinostat) to form the nanodrugs NP-I/P. NP-I/P treatment not only restored the sensitivity of tumor cells to death and diminished tryptophan-kynurenine metabolism but also increased the release...
Circular RNA (circRNA) is a group of endogenous non coding RNA molecules that connect from head to tail through reverse splicing to form a covalent closed loop structure [14]. More and more studies have shown that circRNA plays a crucial role in regulating tumor proliferation and metastasis in...
We also generated a cell-permeable form of the IPMK-DN peptide. Compared to the IPMK 93–182 fragment, the shorter fragment of IPMK 93–126 was shown to efficiently lower PLCγ1 phosphorylation in fibroblasts (fig. S5C). This peptide was fused to the protein transduction domain (PTD), ...
For these studies, we compared the Vybrant DiD (DiD) membrane dye or CellTrace Far Red (CTFR, similar to CFSE), both of which are diluted ~ 50% after each cell division. To assess the durability of each dye, we lethally radiated murine fibroblasts (30 Gy) to halt their ...
a Histogram with quantitative bar plot exhibited and compared the proliferative activity of naïve CD4+ T cells cocultured with monocytes in each group based on CFSE assay. b Contour plots with quantitative bar chart revealed the proportion of CD4+CD25+Foxp3+ Tregs. c Quantitative bar charts ...