适用产品目录号:E2691,E2692,E2693普洛麦格(北京)生物技术有限公司电话:***3,01058256268网址:.promega简易流程图QuickPROTOCOL接种和培养细胞配制转染试剂与质粒DNA复合物复合物加入至培养的细胞中24-48小时后检测转染效果FuGENE®6转染试剂与DNA的比例4:13.5:13:12.5:12:11.5:1加培养基至终体积1100μl100μl100...
CHO 基因表达 Optimized Protocol of a Frequency Domain Fluorescence Lifetime Imaging Microscope for FRET Measurements. 2009 Fugene HD HD-1000 CHO 基因表达 Leukotriene E4–induced pulmonary inflammation is mediated by the P2Y12 receptor. 2009 Fugene HD HD-1000 CHO 基因表达 The unique substrate specific...
Procedures and required materials5 4.1 Before you begin 5 4.2 Factors for successful transfection5 4.3 Standard protocol for transient and stable transfection 8 P 4.3.1 Preparation of cells for transfection 8 R O 4.3.2 Preparation of FuGENE 6 Reagent:DNA complex9 C E 4.3.3 Scale-up for ...
lines. FuGENE®HD interacts with nucleic acids to provide efficient and safe entry into the cell, allowing users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells. Its simple, easy-to-use protocol allows users to free up valuable lab...
lines. FuGENE®HD interacts with nucleic acids to provide efficient and safe entry into the cell, allowing users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells. Its simple, easy-to-use protocol allows users to free up valuable lab...
FuGENE 6 Transfection Reagent is easy to use. By following the standard protocol, high levels of transfection can be obtained with fewer steps than traditional reagents and methods. In some experiments, higher expression levels can be achieved by optimizing several parameters on a cell-line by cell...
已经过Promega 公司验证的、可用FuGENE ®HD 进行高效率、低毒性转染的细胞：以上表格中所列的细胞种类，都可以使用在线工具 http://cn.promega.com/resources/tools/fugene-hd-protocol-database/ 检索专用的操作步骤。输入培养板的类型和转染孔的个数，在线程序会为您自动输出专用于该细胞系的质 粒、转染试剂用量...
This is equivalent to over 6,000wells in a 96-well plate or 1,000 wells in a 24-well plate.L Optimal expression depends upon experimental conditions includ-ing cell type, passage history, confluence, seeding protocol, com-plex incubation time, serum batch, etc. The above amounts ofreagents...
According to a protocol of an FuGENE6 transfection reagent, 8×105 CHO cells were inoculated to a 10-cm-diameter dish on the day before transfection, and the cells were cultured overnight. Thereafter, an expression vector pEF4-sCDH3... H Akihiro,N Akio,W Masahiko,... - CN 被引量: ...
Quick PROTOCOL FuGENE® HD 转染试剂简明操作指导 注意:这是节选操作步骤,详细英文说明书见 适用产品目录号:E2311、E2312 /protocols/ ® FuGENE HD转染试剂采用专利的脂质混合体配方,提供了一个高效、低毒的转染方法;不含任何人体或其他动 物来源的组分,转染前细胞不用去血清培养,转染后不用更换培养基,最大限...