CHO-K1 细胞分析 FuGENE 6 Transfection Reagent: The Gentle Power 2004 Fugene 6 F6-1000 Chondrocytes 基因表达 Comparison of Efficacy of Endogenous and Exogenous IGF-I in Stimulating Matrix Production in Neonatal and Mature Chondrocytes 2015 Fugene 6 F6-1000 Chorionic 基因表达 A Novel Distal Enhancer ...
Procedures and required materials5 4.1 Before you begin 5 4.2 Factors for successful transfection5 4.3 Standard protocol for transient and stable transfection 8 P 4.3.1 Preparation of cells for transfection 8 R O 4.3.2 Preparation of FuGENE 6 Reagent:DNA complex9 C E 4.3.3 Scale-up for ...
Best-in-class DNA Transfection Reagent-NEW! FuGENE®4K: Maximizes DNA transfection efficiency Minimal-to-no cytotoxicity Quick and easy protocol & optimization Request a free sample today to test in your lab! LEARN MORE NEW!– Superior and Gentle siRNA Transfection ...
FuGENE 6 Transfection Reagent is easy to use. By following the standard protocol, high levels of transfection can be obtained with fewer steps than traditional reagents and methods. In some experiments, higher expression levels can be achieved by optimizing several parameters on a cell-line by cell...
Best-in-class DNA Transfection Reagent-NEW! FuGENE®4K: Maximizes DNA transfection efficiency Minimal-to-no cytotoxicity Quick and easy protocol & optimization Request a free sample today to test in your lab! LEARN MORE NEW!– Superior and Gentle siRNA Transfection ...
A protocol for nonliposomal transfection of DNA into a variety of cell lines with high efficiency and low toxicity. Removal of serum or culture medium is not required, nor is washing or changing medium after introducing the reagent/DNA complex.
According to a protocol of an FuGENE6 transfection reagent, 8×105 CHO cells were inoculated to a 10-cm-diameter dish on the day before transfection, and the cells were cultured overnight. Thereafter, an expression vector pEF4-sCDH3... H Akihiro,N Akio,W Masahiko,... - CN 被引量: ...
using3 lofreagentcombinedwith1–2 gDNAperwell.Thisisequivalenttoover6,000wellsina96-wellplateor1,000wellsina24-wellplate.LOptimalexpressiondependsuponexperimentalconditionsinclud-ingcelltype,passagehistory,confluence,seedingprotocol,com-plexincubationtime,serumbatch,etc.TheaboveamountsofreagentsworkwellwithHeLa...
FOR IN VITRO USE ONLY.FuGENE HD Transfection ReagentFor the transient and stable transfection of animal and insect cells®Cat. No. 04 709 691 001Cat. No. 04 709 705 001Cat. No. 04 709 713 001 1)Cat. No. 05 061 369 001 Cat. No. 04 883 560 0011)0.4 ml (up to 120 ...
6 Transfection Reagent is formulated to work at low cell densities, whereas FuGENE? HD Transfection Reagent is formulated to work at higher cell densities. Cells must be healthy and free of mycoplasma and other contaminants. L If you have used FuGENE? 6 Transfection Reagent in the past, we ...