125 µL Opti MEM serum reducing medium (Gibco, Grand Island, NY, USA) containing the required amount of siRNA and Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) was added respectively to two centrifuge tubes and incubated at room temperature for 10 min to form a ...
Frozen tissue sections and cells seeded onto the glass coverslips were incubated with 1 μM C11 BODIPY Reagent (GLPBIO, GC40165, Montclair, CA) for 30 min at 37 °C in the dark. Then cells and sections were rinsed with PBS and fixed at room temperature with 4% paraformaldehyde for...
While planning to buy bedroom furniture, it is important to consider material features of the furniture which have properties that are more suitable to the bedroom environment. Some of these materials include plastic, glass finishes metal or even wood. This will depend on your preference or your ...
Full size image We further explored the relevance of FTO, YTHDF2 and NEDD4. Using western blot, we found that FTO overexpression led to an upregulation of NEDD4, and knockdown of YTHDF2 significantly reduced NEDD4 expression. However, overexpressed FTO could not upregulate NEDD4 expression in...
Within 24 h later from the last habituation session, animals returned to the familiar open field apparatus for 10 min in the presence of two identical objects (glass beaker filled with blue marbles), placed in two diagonal corners of the box (11.5 and 14 cm from each wall respectively, and...
Full size image There was also a positive correlation between circSCMH1 and FTO (Pearson correlation coefficientr = 0.5677,P = 0.0542) (Supplementary Fig.5d). Thus, we first examined the effect of circSCMH1 on the expression of FTO. However, western blotting showed no significant dif...
Plastic or glass cell culture dishes were used to plate PDAC cell lines in DMEM with 10% FBS and penicillin/streptomycin (100 U/mL) in a 5% CO2 incubator at 37 °C (NEST Biotechnology). HPNE, Miapaca-2, Panc-1 and CFPAC-1 cells were kindly provided by Cell Bank/Stem Cell Bank, Ch...
You can also place flowers in glass vases for an extra pop of color. The natural elements will help bring a sense of peace and relaxation to the home. Add Texture Texture is an essential element when it comes to decorating your home. You can add texture by using rugs, throws, cushions,...