125 µL Opti MEM serum reducing medium (Gibco, Grand Island, NY, USA) containing the required amount of siRNA and Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) was added respectively to two centrifuge tubes and incubated at room temperature for 10 min to form a ...
Ferroptosis, a distinctive iron-dependent form of regulated cell death induced by the excessive accumulation of lipid peroxides on cellular membranes, is proved to have a dual role in cancer, leading to tumor cell proliferation or elimination [18]. Understanding the regulation of this iron-dependent...
obesity, the relationship between sirtuins and OP, and a discussion focusing on the possibility of treating OP caused by obesity by targeting sirtuins. This will be based on the common influences on the occurrence of obesity and OP (such as mesenchymal stem cells, gut microbiota, and insulin)....
For instance, using RNC-seq, Zhang’s group identified an 87-amino-acid tumor-suppressive peptide encoded by the circular form of a lncRNA LINC-PINT in glioblastoma cells [51]. However, preserving the integrity of RNC mRNAs during RNC-seq procedures can be technically challenging owing to RNC...
FTO is involved in the regulation of the cell cycle, cell differentiation, splicing, cancer development, immunotherapy, and various other biological functions [35,36,37,38]. Importantly, ALKBH5, as another member of the α-ketoglutarate-dependent dioxygenase family, was found that knocking out ...
These medications later undergo re-oxidation to form disulfide bonds. However, this approach is restricted to drugs soluble in lipids and faces difficulties expanding its application. Furthermore, there are cases where the inclusion of reducing agents can lead to potential toxicity [86]. Thermal ...
transcripts, with implications for gene expression and cellular functions. The distribution and abundance of m6A are tightly controlled by these writers, as well as by “eraser” enzymes such as fat mass and obesity-associated protein (FTO) and AlkB homolog 5 (ALKBH5), which remove the methyl ...
(S1179D) form of eNOS displayed enhanced vascular reactivity, reduced occurrence of severe stroke, and improved CBF compared to mice expressing the non-phosphorylated (S1179A) form of eNOS (Atochin et al.2007). Statins inhibit the Rho/ROCK pathway, which activates the PI3K/Akt pathway. This...
Furthermore, in aged mice, Runx2 engages a histone acetyltransferase p300 and the coactivator NCOA1 (nuclear receptor coactivator 1) to form a complex, thereby transactivating the expression of 14 MMPs (MMP1b, -2, -3, -7, -8, -9, -10, -12, -13, -15, -16, -23, -27, and ...
A novel mechanism of FTO modulating the progression of endometriosis through mediating the m6A methylation of GEF-H1 in a YTHDF1-dependent manner Xin-Yu Ding, Hua-Ying Zhang, Jia-Hao Chen, Meng-Jie Yang, Zhi-Xiong Huang, Yi-Hong Lei, Qin-Kun Sun, Jian-Bin Bai, Dian-Chao ...