Furthermore, FTO, and indeed m6A, may also be associated with Parkinson’s disease (PD), as dopaminergic signaling is negatively affected upon the inactivation of FTO [52]. More recently, another study looked more closely into the role of m6A in PD [53]. They modeled the disease in rats...
125 µL Opti MEM serum reducing medium (Gibco, Grand Island, NY, USA) containing the required amount of siRNA and Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) was added respectively to two centrifuge tubes and incubated at room temperature for 10 min to form a ...
m6A plays a pivotal role in controlling vital downstream molecules and critical biological pathways, contributing to the pathogenesis and evolution of numerous conditions. This review provides an overview of m6A demethylases, explicitly detailing the structural and functional characteristics of FTO and ALKB...
Polycystic ovary syndrome (PCOS), a common endocrine disorder in women of reproductive age, is closely associated with chronic low-grade inflammation and metabolic disturbances. In PCOS mice, dietary inulin has been demonstrated to regulate intestinal flora and inflammation. However, the efficacy of di...
In addition, miRNAs also regulate apoptosis, a form of programmed cell death, may be triggered by common upstream signals [115]. Li et al. found that the overexpression of miRNA-543-3p in LPS-treated PDLSCs, increasing the expression of Bcl-2, while decreasing the expression of Bax, cleave...
For instance, using RNC-seq, Zhang’s group identified an 87-amino-acid tumor-suppressive peptide encoded by the circular form of a lncRNA LINC-PINT in glioblastoma cells [51]. However, preserving the integrity of RNC mRNAs during RNC-seq procedures can be technically challenging owing to RNC...
B Expression level of FTO mRNA in gemcitabine-resistant and -sensitive cells. C The findings of the CCK-8 proliferation experiment showed that inhibiting FTO expression reduced the growth of gemcitabine-resistant cells. D The ability of CFPAC-GM and Colo357-GM cells to form colonies was ...
The “writers” include METTL3, METTL14, WTAP, RBM15, KIAA1429, and ZC3H13; the “erasers” mainly consist of ALKBH5 and FTO; the “readers” are primarily composed of YTHDF1/2/3, YTHDC1/2, IGF2BP1/2/3, HNRNPA2B1, and HNRNPC [5, 8]. This article will review the latest ...
RNA modifications significantly contribute to immune evasion, indirectly promoting drug resistance [285,286,287,288]. Studies have shown that FTO enhances the self-renewal and immune evasion capabilities of cancer stem cells (CSCs) [289,290,291]. Reducing FTO expression through genetic manipulation or...
The m6A modified demethylase FTO affects the progression of gastric cancer (GC), and the role mechanism of FTO in GC is still unclear. We, here, explored the role of FTO and unrevealed the mechanisms of its function in GC. The expression and clinical pro