进一步分析质谱结果发现,FSP1除了可以利用其催化活性产生FADH2(用于还原COQ10和Vitamin K)外,还可以产生另外一种中间代谢产物6-OH-FAD,并发现补充6-OH-FAD能够有效抑制肿瘤细胞发生铁死亡。有趣的是,在FSP1 缺失的细胞中补充6-OH-FAD依...
FSP-1作为一种与铁死亡密切相关的蛋白，通过FSP1-CoQ10-NAD(P)H这种独立于谷胱甘肽(GSH)-GPX4的途径，将脂质过氧化物还原为无毒脂醇，从而抑制铁死亡的发生。FSP-1的检测能够揭示细胞铁死亡的调控机制，为多种疾病研究提供新的策略和方法，从而推动生命科学及医学的进步和发展。Elabscience®自主研发了全球首款检测...
此外,研究者进一步研究发现,FSP1抑制铁死亡的主要机制是利用NAD(P)H还原CoQ10,抑制脂质过氧化,进而抵抗铁死亡发生。药物靶向FSP1与GPX4抑制剂协同作用可诱发一系列肿瘤细胞的铁死亡。研究结论:FSP1-CoQ10-NAD(P)H信号通路作为独立的系统,与G...
Fsp1-CoQ10-NAD(P)H pathwayStudies have confirmed that elevated glucose levels could lead to renal fibrosis through the process of ferroptosis. Liraglutide, a human glucagon-like peptide-1 (GLP-1) analogue, is a potential treatment option for diabetes. This study aimed to examine the potential ...
(P)H pathway suppressed lipopolysaccharide-induced ferroptosis in HK-2 cells. Ginsenoside Rg1 alleviated ferroptosis in HK-2 cells by regulating the ferroptosis suppressor protein 1-CoQ10- NAD(P)H pathway. Moreover, ginsenoside Rg1 regulated the ferroptosis suppressor protein 1-CoQ10-NAD(P)H ...
Can small molecular drugs targeting the CoQ10 synthesis pathways (such as the mevalproate pathway) or NAD(P)H generation pathways (such as the PPP) be selected to interfere with the glutathione-independent FSP1-CoQ10-NAD(P)H ferroptosis inhibition axis? Can circRNAs directly bind to FSP1, o...
published a study demonstrating that the FSP1-CoQ10-NAD(P)H pathway regulates ferroptosis in a manner independent of GPX4 signalling [48]. Interestingly, Dai et al. further found that FSP1 blocks ferroptosis independent of CoQ10/ubiquinol metabolism [49]. Notably, erastin directly binds VDAC2 ...
NAD(P)H and defends against ferroptosis through its oxidoreductase activity, and why only FSP1 in AIF family plays a key role in suppressing ferroptosis. Exploring the molecular mechanisms of FSP1 catalytic activity will be beneficial for developing therapeutics that acts through this FSP1-CoQ10...
In conclusion, the FSP1–CoQ10–NAD(P)H pathway exists as a stand-alone parallel system, which co-operates with GPX4 and glutathione to suppress phospholipid peroxidation and ferroptosis. This is a preview of subscription content, access via your institution Access options Access Nature and 54 ...
4.验证FSP1与NADK的相关性 临床样本分析显示NADK与FSP1表达水平呈中度正相关(r=0.572,p<0.01);在野生型肺腺癌细胞中补充NADPH能增强FSP1的表达;在敲降NADK的肺腺癌细胞系中FSP1表达水平下降,补充NADPH可恢复FSP1的表达水平. 5.NADPH对FSP1的底物CoQ10和产物CoQ10H_2的影响 敲降NADK导致FSP1底物CoQ10水平上升...