3. DSB & template-free editing in bacteria 4. DSB & template-free editing in plants 5. Applications: current & future 6. Concluding remarks, challenges & prospects Declaration of competing interest Acknowledgments ReferencesShow full outline Cited by (33) Figures (3) Tables (2) Table 1 Table...
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(LTB) could be fluorescently labeled using an LTB-Strep fusion protein, which is a proof-of-concept for future Trojan horse applications. Further, we performed a mutational analysis of the CtxA subunit as its template was modified, and an amber stop codon was inserted into CtxA’s active ...
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Fourier transform infrared (FTIR) spectra revealed the presence of signature absorptions at 1670 cm−1 (CO stretching of PVP) and 1285 cm−1 (C–N stretching of PVP) [36], thereby confirming successful incorporation of PVP within the as-prepared ZnO2 NPs (Fig. S2). To endow ZnO2 ...
In addition, an orthogonal cell-free translation system is presented that enabled the site-directed incorporation of p-Azido-L-phenylalanine by amber suppression. Functionality of cell-free synthesized receptor molecules is demonstrated by investigation of autophosphorylation activity in the absence of ...
(minkc−at1/ μKMm −1) 2 0.002 0.0015 10 0.1 0.0009 0.1 aEfficiency relative to T opposite same template 1 ↓103 ↓7 × 104 — 1 ↓100 ↓9 × 105 ↓100 Table 1. Steady-state kinetic parameters of nucleotide incorporation opposite...