Article metricsAbstract Given continuous improvements in industrial production and living standards, the analysis and detection of complex biological sample systems has become increasingly important. Common complex biological samples include blood, serum, saliva, and urine. At present, the main methods used...
itruns offthe DNA template and releases the transcript, thereby ensuring the process is performed several times (Fig.2a). In this way, milligram quantities of RNA can be produced per milli
To confirm these individual cell line results, we constructed independent triplicate libraries of the pooled Sample A using three of the original four WES kits (Roche MedExome, IDT xGen, Agilent SureSelect) and sequenced each library very deeply (deduplicated average depth of 180–580× depending...
Field-portable chip-based CE systems have been reported in the literature with improved dimensions and weight, mainly due to the incorporation of small high-volt- age power supplies and on-chip detectors. For instance, the first portable chip-based CE system showed highly reduced dimensions (102...
While this method allows the incorporation of platform-specific adaptors and, if required, barcodes for multiplexing during the PCR [28], it may introduce systematic biases, as well as stochastic error [29–32]. Small amplicon-based approaches cannot readily be extended to full genome sequencing,...