According to the nonprofit organizationAsistencia al Celíaco de México,the prevalence of celiac disease in Mexico is approximately 1 per 150 people. Population-wide studies estimate thatapproximately 800,000 Mexicans had celiac disease as of October 2018, though as with many countries the condition i...
To generate the HeLa EGFP-HURP cell line, HeLa cells (ATCC; CCL-2) were co-transfected with the pCMV-Cas9-RFP plasmid (Sigma-Aldrich, encoding for both Cas9 gene and the guide RNA targeting the first exon of the humanDLGAP5gene) along with the EGFP-HURP repair template cloned into the...
Defective DNA repair in FA cells leads to G2 phase cell cycle arrest and increased cell death in response to DNA cross-linking reagents, which may contribute to the manifestation of FA disease phenotypes1. Patients with biallelic mutations in any of the FANC genes frequently succumb to BMF, ...
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Combined with selection marker free knockout repair DNA fragments, a set of the seven single knockout strains was obtained through homology directed repair (HDR) with an average efficiency of 90%. Cas9–sgRNA plasmids could effectively be cured by removing selection pressure, allowing the use of ...
SMATemplateGroupId Extended Data Type [AX 2012] SMATemplateGroupIdParent Extended Data Type [AX 2012] SMATimeAgreementId Extended Data Type [AX 2012] SMAWIPSubscription Extended Data Type [AX 2012] SMAWIPSubscriptionLedDimDefAc Extended Data Type [AX 2012] SMAWorker Extended Data Type [AX 2012...
Cell-free DNA (cfDNA), present in circulating blood plasma, contains information about prenatal health, organ transplant reception, and cancer presence and progression. Originally developed for the genomic analysis of highly degraded ancient DNA, single-
A model-based approach is developed to estimate the distribution of time from seroconversion to diagnosis with acquired immunodeficiency syndrome (AIDS) as a function of selected time-dependent covariates. The approach is applied to longitudinal data collected over 4 years of follow-up from 450 men ...
This is certainly of dramatic interest for fundamental investigations addressing the ability of proteins to locate specific sites or structures among a vast excess of non-specific, intrinsically bent DNA, as in the relevant case of mismatch repair proteins interrog- ating DNA to find out ...
There was no impact on mutant survival compared to control, suggesting that OTUD6 does not regulate DNA break repair (Fig. 1d). We also used a genetic method that reports the types of double-stranded DNA repair events19. With this assay we detected a small decrease in homology-directed ...