cre的早期表达始于E8.5-E9.0的端脑。 在其他头部区域也观察到某些表达,例如耳囊、嗅觉斑、视囊前半部、咽内胚层、以及前肠的E10.5。 人类同源基因 FOXG1 品系描述 将Cre-WPRE-polyA插入到小鼠Foxg1基因起始密码子处。 应用领域:Cre工具鼠,端脑,前视泡,耳泡,面部和头部外胚层,嗅上皮中后脑和咽囊的交界处...
ASD的社会表型产生于兴奋性和抑制性神经元中FoxG1的减少或增加 FoxG1功能获得可以破坏锥体神经元的迁移,因此,选择在出生后早期(出生后第一周),大多数神经元迁移完成后,增加FoxG1水平,利用前脑中兴奋性和抑制性谱系特异性的Cre驱动因子调控FoxG1表达,发现只...
RNA-Seq analyses of Foxg1cre/+ hippocampi and N2a cells overexpressing miR200 family members identified cAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) as a target of miR200 in neural cells. PRKAR2B inhibits postsynaptic functions by attenuating protein kinase A (PKA) ...
Edn1fl/fl;Foxg1-Cre Embryos Display Similar Craniofacial Defects with Edn1-/- EmbryosE. GARCIA
本发明属于生物技术领域,具体涉及阿尔茨海默病病变区FoxG1过表达小鼠模型的构建方法及应用。本发明将FoxG1转基因小鼠与海马、皮层及纹状体特异脑区表达Cre重组酶的Cre工具小鼠交配,获得海马、皮层及纹状体FoxG1条件性过表达小鼠,再与阿尔茨海默病模型小鼠交配得到阿尔茨海默病病变脑区FoxG1条件性过表达小鼠。他莫昔芬...
尽管中间神经元在调节皮层功能中起着关键性的作用,但对于皮层中间神经元的发育调控机制目前还知之甚少。本课题探讨转录因子Foxg1在中间神经元发育过程中的作用。通过将Foxg1fl/fl和Dlx5/6-Cre小鼠进行交配从而在腹侧端脑条件性敲除Foxg1基因,结果导致中间神经元发育的异常。在发育中的中间神经元中敲除了 Foxg1后...
(h,i:Nex-Cre;Dlx-Cre;FoxG1-C/ +, scheme inl). In the Heterozygous model, the cortical thickness of the S1 Barrel (g) but not the mPFC (f) is reduced compared to the Control (d,e). Removal of one copy ofFoxG1selectively in postmitotic neuronal populations rescues the ...
48 h later, CRE protein expressing cells were enriched by FACS sorting (FACS-Aria, BD-Biosciences) followed by replating at a density of 300 cells/cm2 in Y27632 containing E8 medium. Individual colonies were picked and PCR genotyped as described above. Off-target analysis on SMASh ...
Disruption of Foxg1 expression by knock-in of Cre Recombinase Effects on the development of the mouse telencephalon 文档格式: .pdf 文档大小: 3.51M 文档页数: 30页 顶/踩数: 0/0 收藏人数: 0 评论次数: 0 文档热度: 文档分类: 论文--期刊/会议论文 ...