FOXD3-AS1miRNAEMT目的:胶质母细胞瘤(GBM)是胶质瘤中最具恶性和侵袭性的组织类型,尽管目前的治疗手段得到很大的发展,但患者的5年生存率不超过5%.故针对胶质瘤分子靶向治疗的研究就显得尤为重要.近些年的大量报道表明,Lnc RNA以及miRNA的异常表达在肿瘤的进展中起着不可忽视的作用.本实验主要讨论了FOXD3-AS1/miR-...
FOXD3-AS1 is downregulated in NB tissues and cell lines, and ectopic expression ofFOXD3-AS1induces neuronal differentiation and decreases the aggressiveness of NB cellsin?vitroandin?vivo. Mechanistically, as a nuclear lncRNA, FOXD3-AS1 interacts with poly(ADP-ribose)?polymerase 1 (PARP1) to...
用qRT -PCR 法检测肝脏组织和血清中lnc -FOXD3-AS1的表达,western blot 和ELISA 法检测大鼠肝脏组织和血清Hepcidin 以及SMAD1/5/8蛋白的表达和激活㊂结果:IDA 组的大鼠IDA 模型诱导成功㊂与对照组比,IDA 组中大鼠肝脏组织和血清中铁含量维持在缺铁状态(均P <0.05),且Hepcidin ㊁lnc -FOXD3-AS1和SMAD...
In addition, E74 like ETS transcription factor 1 (ELF1) stimulated FOXD3-AS1 transcription, and ELF1 silence-suppressed malignant phenotypes of OS cells were offset by FOXD3-AS1 upregulation. Overall, present work elucidated that ELF1-activated FOXD3-AS1 aggravated cell migration, invasion and...
目的 探究FOXD3-AS1与miR-135a-5p的相互作用及对肾细胞癌(RCC)ACHN细胞增殖,迁移和侵袭的影响.方法 选取本院2017年1月—2019年12月RCC手术切除癌组织和癌旁组织60份,RT-qPCR检测组织和细胞中FOXD3-AS1和miR-135a-5p表达水平;ACHN细胞分别或联合转染si-NC,si-FOXD3-AS1-1,si-... 查看全部>>关键...
【目的】 1.验证LncRNAFOXD3-AS1在正常人乳腺上皮细胞株MCF10A和人乳腺癌细胞株(BT-549,MDA-MB-231,MCF-7)中的表达. 2.探讨敲低LncRNAFOXD3-AS1的表达后乳腺癌细胞生物学功能的变化. 3.初步探讨LncRNAFOXD3-AS1对乳腺癌细胞的作用机制. 【方法】 采用生物信息学的方法对肿瘤基因组图谱(Thecancergenome...
Risk-Associated Long Noncoding RNA FOXD3-AS1 Inhibits Neuroblastoma Progression by Repressing PARP1-Mediated Activation of CTCF Molecular therapy : the journal of the American Society of Gene Therapy (2018) -4Comments pubmed: 29398485 doi: 10.1016/j.ymthe.2017.12.017 issn: 1525-0016 issn: 1525...
长链非编码RNA FOXD3-AS1肿瘤细胞分子机制长链非编码RNA(LncRNAs)是一组长度大于200个核苷酸的非编码RNA.因为缺少明显的开放阅读框,LncRNAs的蛋白质编码能力非常有限或无编码能力,但越来越多的研究报道表明,LncRNAs在肿瘤的复杂调控网络中有很大的作用,如肿瘤的增殖,侵袭等.LncRNA叉头盒D3反义RNA1(FOXD3-AS1),由...
Overexpression of FOXD3-AS1 antagonized the inhibitory impact of miR-765 on the expression of BCL2L13 and the apoptosis of N2a cells treated with OGD/R, while FOXD3-AS1 knockdown promoted the inhibitory impact of miR-765 on the expression of BCL2L13 and the apoptosis of N2a cells ...
These findings demonstrate that overexpression of lncRNA FOXD3-AS1 aggravates myocardial I/R injury through promoting autophagy, which was regulated by activating NF-κB/iNOS/COX2 signaling. 展开 关键词: FOXD3-AS1, cardiomyocytes, ischemia/reperfusion, apoptosis, autophagy ...