Some of these roles, such as protein folding, protein stability and efficient intracellular transport, are more dependent simply on the presence of a saccharide, rather than on its exact structure. Other functions are directly related to specific saccharide structures. This article focuses on the ...
Using motion planning to study protein folding pathways. Our focus in this work is to study the protein folding mechanism assuming we know the native fold. That is, instead of performing fold prediction, we ... NM Amato,G Song 被引量: 345发表: 2002年 Structures of Oligosaccharide-Bound For...
We use the h-principle for folding maps (a theorem of Eliashberg) and the h-principle for symplectic forms on open manifolds (a theorem of Gromov) to show that, for orientable even-dimensional manifolds, the existence of a stable almost complex structure is necessary and sufficient to warrant...
Given its ability to bind to polyubiquitin, the UBA domain may be needed for the formation of cytoplasmic bodies. In support of this, we found that a construct lacking the UBA domain (p62(1–385)) and the I431A mutant, which affects the folding of the UBA domain, both showed a diffuse...
A complete theory of ligand discrimination would be able to describe the types of motions and energetic connections that define selectivity for a particular ligand within a particular system. In molecular simulations, binding calculations usually focus on a circumscribed region of the protein near the ...
Previously, we have introduced a method whereby novel disulfide side chains can be produced in the interior of a protein by modifying a cysteine residue after denaturant-induced unfolding [Wynn, R., & Richards, F. M. (1993) Proteins: Struct., Funct., Genet. 2, 395-403]. Here the disu...
α-Tropomyosin (Tm) is a two-stranded α-helical coiled-coil protein, which participates in the regulation of muscle contraction. Unlike Tm purified from vertebrate muscle, recombinant Tm expressed in Escherichia coli is not acetylated at the N-terminal residue and loses the capacity to undergo he...
Protein Structure and FoldingStructural Differences between Active Forms of Plasminogen Activator Inhibitor Type 1 Revealed by Conformationally Sensitive Ligands* Author links open overlay panelShih-Hon Li ‡ § ¶ 1, Natalia V. Gorlatova ∥, Daniel A. Lawrence **, Bradford S. Schwartz ‡ §...
Protein folding in cells is regulated by networks of chaperones, including the heat shock protein 70 (Hsp70) system, which consists of the Hsp40 cochaperone and a nucleotide exchange factor. Hsp40 mediates complex formation between Hsp70 and client proteins prior to interaction with Hsp90. We use...
HSP70 is a multifunctional protein involved in many cell processes, such as protein folding, assembling and deassembling of multimeric protein complexes, protein transport, and translocation across cell and mitochondrial membranes. It is believed thatproteins of the HSP70 family serve as molecular chapero...