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The apoptosis of cancer cells was measured using the Annexin V-FITC/PI Kit (catalog KGA1102, KeyGEN) and flow cytometry analysis in accordance with the manufacturer’s instructions in order to determine the levels of cell apoptosis. In vitro cytotoxicity assay Peripheral blood mononuclear cells (...
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operating above 30o C [86o F], a fan fault or executing workloads with extensive use of heavy instructions sets like Intel® Advanced Vector Extensions 512 (Intel® AVX-512), may assert thermal and/or performance faults with an associated event recorded in the System...
Brains of the desired age were dissected and stained for EdU presence using the Click-iT Plus EdU detection kit of Thermo Fisher (# C10638) according to the instructions of the manufacture. Dye injection assay Brains of wandering L3 larvae were dissected on ice and immediately placed on Poly-...
Organs were weighed and homogenized by bead dissociation using a MagNA Lyzer (Roche) in a volume of DMEM containing 2% FBS. Viral RNA was isolated from tissue homogenates using the RNeasy Mini kit (Qiagen), as per the manufacturer’s instructions. ZIKV RNA levels were determined by TaqMan one...
Each lab is complete with a quick-start guide, detailed instructions, background information, and one or more guided exercises. To see how iNSIGHT works, try the FREE iNSIGHT Color Mixing app. A printable PDF Student Guide that contains the exercises and other supporting materials, and a ...
The immune-enriched subtype, which displayed a higher infiltration of immune cells such as CD8+ T cells and M1 macrophages, in conjunction with a higher tumor mutational burden, presented with a better prognosis. According to the Kraken2 pipeline's findings, the microbiome profiles demonstrated ...
Mouse IL-2 production and mouse mast cell protease-1 (mMCP-1) in sera were detected by using the respective ELISA kits (eBioscience, San Diego, CA) in accordance with the manufacturer’s instructions. ChIP assay The antibodies used for chromatin immunoprecipitation (ChIP) assay were anti-...