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Table 2 Instructions and items of the Italian version of the SCS-SF Full size table Self-Compassion We employed the SCS-SF (Raes et al., 2011; response scale from 1 = almost never to 5 = almost always). Because results of the Aim 1 section of the present paper supported a...
Brains of the desired age were dissected and stained for EdU presence using the Click-iT Plus EdU detection kit of Thermo Fisher (# C10638) according to the instructions of the manufacture. Dye injection assay Brains of wandering L3 larvae were dissected on ice and immediately placed on Poly-...
Organs were weighed and homogenized by bead dissociation using a MagNA Lyzer (Roche) in a volume of DMEM containing 2% FBS. Viral RNA was isolated from tissue homogenates using the RNeasy Mini kit (Qiagen), as per the manufacturer’s instructions. ZIKV RNA levels were determined by TaqMan one...
RNA was extracted from cell pellets using the RNeasy kit (Qiagen) according to the manufacturer’s instructions. Complementary DNA was generated using equal amounts of RNA from each sample and the iScript complementary DNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions. Reac...
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Transcribed RNAs were then purified by micro Bio-Spin 6 chromatography columns (Bio-rad) according to the manufacturer’s instructions. The sizes of RNAs were checked by gel electrophoresis on a denaturing 6% polyacrylamide gel. 10 pM (3000 CPM) of labeled RNA was incubated at 90°C for 5 ...
See prescribing information for preparation instructions, dosage, and administration. Storage: Refrigerate at 2°C to 8°C (36°F to 46°F). Unopened vial can be stored up to 30°C(86°F) for a single period of up to 3 months but not exceeding the expiry date. Reconstitute with 20 mL...