Single-cell libraries were generated following the manual instructions of 10X Genomics (Chromium Single Cell 3’ Reagent Kits, V3.1). The libraries were sequenced either on an Illumina Novaseq platform at Novogene Co., Ltd to generate 150-bp paired-end reads (day 6 blastoids) or on MGISEQ...
Single-cell libraries were generated following the manual instructions of 10X Genomics (Chromium Single Cell 3’ Reagent Kits, V3.1). The libraries were sequenced either on an Illumina Novaseq platform at Novogene Co., Ltd to generate 150-bp paired-end reads (day 6 blastoids) or on MGISEQ...
1139-1153 Google Scholar 59 S. He, N.E. Sharpless Senescence in health and disease Cell, 169 (2017), pp. 1000-1011 View PDFView articleGoogle Scholar 60 J. Chang, Y. Wang, L. Shao, et al. Clearance of senescent cells by ABT263 rejuvenates aged hematopoietic stem cells in mice ...
To introduce point mutations in human Cx46 that change Cys residues into Ala residues, site-directed mutagenesis was performed using the Quikchange II XL kit (Agilent Technologies, Santa Clara, CA, USA) in accordance with the instructions of the manufacturer. Separate mutagenesis reactions were perf...
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The level of skin barrier impairment was assessed daily during each assessment cycle by transepidermal water loss measurements using a handheld vapometer (Delfin Technologies, Kuopio, Finland), following the manufacturer’s instructions. The level of skin erythema was measured daily during each assessment...
or more intuitive gesture operations, making it easier for users to start, (c) introduce a detailed tutorial mode that enables novice users to gradually learn how to use the tools and functions through interactive step-by-step instructions, (d) provide more personalized setting options, allowing ...