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3.5 × 104 cells were resuspended in 1 ml of 5iLAF-I medium and seeded into one well of an AggreWell 400 24-well plate (Stemcell,34411) pretreated with anti-adherence rinsing solution (Stem Cell Technologies, 07010) following the instructions and cultured under 20% O2 and 5% CO2 at 37...
Candidates are to follow the instructions provided by the Test Centre staff. JEE Advanced AAT Admit Card 2025 The authorities do not publish a separate admit card for JEE Advanced AAT. The candidate has to produce their JEE Advanced 2025 admit card at the exam venue for the Architecture ...
3.5 × 104 cells were resuspended in 1 ml of 5iLAF-I medium and seeded into one well of an AggreWell 400 24-well plate (Stemcell,34411) pretreated with anti-adherence rinsing solution (Stem Cell Technologies, 07010) following the instructions and cultured under 20% O2 and 5% CO2 at 37...
These mixtures were transfected using Lipofectamine 2000 according to manufacturer’s instructions. Luciferase assays were carried out as above. Expression levels of depleted and transfected proteins were checked by Western blotting. To test the influence of HIV1-Tat on the HIV1-TAR reporter, cells ...
Installation Instructions Euro formwork system sccessories Euro formwork is very popular for wall concrete construction. Conveniently located handles make set up, stripping, and handling easier. With proper care, contractors can expect that forms will be able to be reused up to 200 times be...
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For quantitative PCR analysis, 10 ng of the cDNA were mixed, with the oligonucleotide sequences used as primers and the LightCycler 480 SYBR Green I Master Mix (Roche, Basel, Switzerland), following the manufacturer’s instructions. The reaction was performed on the LightCycler®480 II (Roche...