Substitutions at Lys13, a surface residue which contributes to a patch of positive charge characteristic of the nitrogenase flavodoxins, had no major effect on stability or function. However, most substitutions at Thr14, which is predicted to hydrogen bond to the phosphate of the prosthetic group...
The SlLLP recombinant protein is expressed as a fusion protein with His-Patch thioredoxin at the N terminus and His6 at the C terminus. E. coli JM109 having pRARE2LysS (Novagen, Madison, WI) that supplies tRNAs for rare codons to enhance the expression of eukaryotic proteins was used for...
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