To characterize the properties of flavin binding and photocycle of SlLLP, a fusion protein of SlLLP with His-Patch thioredoxin at the N terminus and His6 at the C terminus was expressed in E. coli. The His-Patch thioredoxin and His6 were fused to facilitate the purification of the fusion...
The monomeric structure of CpsUbiX is stabilized by two different patches of inter-subunit contacts, namely patches 1 and 2, to generate a dodecamer. Patch 1 is formed by inter-helical hydrophobic interactions involving three hydrophobic residues, Leu150, Met153 and Leu154, at the α7-helix of...